Anti-CCR2 antibody [EPR23494-47] (ab254375)
![Anti-CCR2 antibody [EPR23494-47] (ab254375)](https://www.abcam.com/ps/products/254/ab254375/Images/ab254375-4-anti-ccr2-antibody-epr2349447-flow-cytometrythp1.jpg "Anti-CCR2 antibody [EPR23494-47] (ab254375)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23494-47] to CCR2
- Suitable for: ICC/IF, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-CCR2 antibody [EPR23494-47]
See all CCR2 primary antibodies -
Description
Rabbit monoclonal [EPR23494-47] to CCR2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC/IF: Human PBMC and THP-1 cells. Flow Cyt: Human PBMC and THP-1 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23494-47 -
Isotype
IgG -
Research areas
Images
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Flow Cytometry - Anti-CCR2 antibody [EPR23494-47] (ab254375)
Flow cytometric analysis of THP-1 (Human monocytic leukemia monocyte) cells labelling CCR2 with ab254375 at 1/600 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Gated on viable cells.
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![Immunocytochemistry/ Immunofluorescence - Anti-CCR2 antibody [EPR23494-47] (ab254375)](https://www.abcam.com/ps/products/254/ab254375/Images/ab254375-2-anti-ccr2-antibody-epr2349447-immunocytochemistry-thp1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CCR2 antibody [EPR23494-47] (ab254375)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized THP-1 cells labelling CCR2 with ab254375 at 1/1000 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing membranous and cytoplasmic staining in THP-1 cell line. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
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![Flow Cytometry - Anti-CCR2 antibody [EPR23494-47] (ab254375)](https://www.abcam.com/ps/products/254/ab254375/Images/ab254375-3-anti-ccr2-antibody-epr2349447-flow-cytometry-human-peripheral-blood-mononuclear-cell.jpg)
Flow Cytometry - Anti-CCR2 antibody [EPR23494-47] (ab254375)Flow cytometric analysis of Human peripheral blood mononuclear cell (PBMC) cells labelling CCR2 with ab254375 at 1/600 dilution (0.1ug) (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left). Cells were stained with rabbit IgG (Left) or ab254375 (Right). Then stained with anti-CD14 conjugated to Alexa Fluor 647.
Gated on viable cells.
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![Immunocytochemistry/ Immunofluorescence - Anti-CCR2 antibody [EPR23494-47] (ab254375)](https://www.abcam.com/ps/products/254/ab254375/Images/ab254375-1-anti-ccr2-antibody-epr2349447-immunocytochemistry-human-pbmc.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CCR2 antibody [EPR23494-47] (ab254375)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Human PBMC cells labelling CCR2 with ab254375 at 1/1000 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing membranous and cytoplasmic staining in Human PBMC cells. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
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![Anti-CCR2 antibody [EPR23494-47] (ab254375)](https://www.abcam.com/ps/products/254/ab254375/Images/ab254375-5-benefits-of-recombinant-antibodies.png)
Anti-CCR2 antibody [EPR23494-47] (ab254375)
