Anti-GSTA1 antibody (ab53940)
Key features and details
- Goat polyclonal to GSTA1
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-GSTA1 antibody
See all GSTA1 primary antibodies -
Description
Goat polyclonal to GSTA1 -
Host species
Goat -
Specificity
The antibody is known to be class-specific and recognizes alpha-form GSTs from rat, mouse, hamster, rabbit, and human. Recognizes GST A1-1(Ya1), A2-2 (Ya2,Yc2), A3-3 (Ya3,Yc), A4-4 (Yk). -
Tested applications
Suitable for: IHC-P, ICC/IF, WBmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Full length native protein (purified) corresponding to Rat GSTA1.
Database link: P00502 -
General notes
Previously labelled as Glutathione S Transferase alpha 1.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
IgG fraction -
Purification notes
IgG fraction was prepared from the antisera using the caprylic acid precipitation method. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-GSTA1 antibody (ab53940)Lane 1. Molecular Weight Marker
Lane 2. GST α Standard
A 1:2000 dilution is recommended for ECL method with overnight incubation at room temperature. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSTA1 antibody (ab53940)A. Negative control (no antibody).
B. Rat liver section stained with ab53940 at 1/1000 dilution followed by anti-goat IgG-HRP with DAB HRP substrate.
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Immunocytochemistry/ Immunofluorescence - Anti-GSTA1 antibody (ab53940)ICC/IF image of ab53940 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53940, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 donkey anti-goat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
