Anti-Calnexin antibody (ab10286)
Key features and details
- Rabbit polyclonal to Calnexin
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Calnexin antibody
See all Calnexin primary antibodies -
Description
Rabbit polyclonal to Calnexin -
Host species
Rabbit -
Specificity
Recognizes ER membrane, mitochondria and cis-Golgi -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
His-Tag E. coli expressed N-terminal ER luminal domain of canine calnexin
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Calnexin antibody (ab10286)
Western blot probed with ab10286 at 1/1000.
Lane 1 – calnexin deficient human NKR T-lymphoblastoid leukemia cell lines.
Western blot probed with ab10286 at 1/1000. Lane 1 – calnexin deficient human NKR T-lymphoblastoid leukemia cell lines. Lane 2 - human CEM T-lymphoblastoid leukemia cell lines.
Lane 2 - human CEM T-lymphoblastoid leukemia cell lines. -
Western blot - Anti-Calnexin antibody (ab10286)Anti-Calnexin antibody (ab10286) at 1/1000 dilution + MCF7 cells
Secondary
Goat anti rabbit at 1/10000 dilution
Predicted band size: 67 kDa
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Western blot - Anti-Calnexin antibody (ab10286) This image is courtesy of an Abreview submitted by Dr Neil TaylorAll lanes : Anti-Calnexin antibody (ab10286) at 1/4000 dilution
Lane 1 : HeLa (Total cell lysate)
Lane 2 : HeLa (Membrane fraction)
Lane 3 : HeLa (Cytoplasmic fraction)
Lane 4 : MDA-MB231 (Total cell lysate)
Lane 5 : MDA-MB231 (Membrane fraction)
Lane 6 : MDA-MB231 (Cytoplasmic fraction)
Secondary
All lanes : HRP conjugated donkey anti-rabbit
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 67 kDa
Observed band size: 67 kDa
Exposure time: 5 minutes
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Western blot - Anti-Calnexin antibody (ab10286) This image is courtesy of an anonymous Abreview.Anti-Calnexin antibody (ab10286) at 1/2000 dilution + HeLa whole cell lysate
Secondary
Goat anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 67 kDa
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Western blot - Anti-Calnexin antibody (ab10286) This image is courtesy of an Abreview submitted by Mr. Yannick Waumans.All lanes : Anti-Calnexin antibody (ab10286) at 1/4000 dilution
Lane 1 : CHO membrane fraction
Lane 2 : CHO cytosolic fraction
Secondary
All lanes : HRP-conjugated Goat anti-Rabbit Ig at 1/5000 dilution
Predicted band size: 67 kDa
