Anti-IDH1 antibody [C12] (ab239606)
Key features and details
- Mouse monoclonal [C12] to IDH1
- Suitable for: WB, IHC-P
- Reacts with: Human, Pig
- Isotype: IgG2a
Overview
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Product name
Anti-IDH1 antibody [C12]
See all IDH1 primary antibodies -
Description
Mouse monoclonal [C12] to IDH1 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human -
Immunogen
Recombinant fragment corresponding to Human IDH1 aa 50-350. N-terminal His tag. Expressed in E. coli.
Database link: O75874 -
Positive control
- WB: Recombinant human IDH1 protein. Human and pig liver tissue lysate. IHC-P: Human stomach, prostate, glioma and kidney tissue.
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General notes
This product was previously labelled as Isocitrate dehydrogenase
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein A/G purified -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
C12 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Images
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All lanes : Anti-IDH1 antibody [C12] (ab239606) at 3 µg/ml
Lane 1 : Human liver tissue lysate
Lane 2 : Pig liver tissue lysate
Secondary
All lanes : HRP-Rabbit Anti-Mouse IgG at 1/5000 dilution
Predicted band size: 47 kDa
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Formalin-fixed, paraffin-embedded human stomach tissue stained for IDH1 with ab239606 at 30 µg/ml in immunohistochemical analysis. DAB staining.
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Formalin-fixed, paraffin-embedded human kidney tissue stained for IDH1 with ab239606 at 30 µg/ml in immunohistochemical analysis. DAB staining.
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Formalin-fixed, paraffin-embedded human glioma tissue stained for IDH1 with ab239606 at 30 µg/ml in immunohistochemical analysis. DAB staining.
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Formalin-fixed, paraffin-embedded human prostate tissue stained for IDH1 with ab239606 at 30 µg/ml in immunohistochemical analysis. DAB staining.
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Anti-IDH1 antibody [C12] (ab239606) at 5 µg/ml + Recombinant human IDH1 protein
Predicted band size: 47 kDa