Antibodies, Proteins, Kits and Reagents for Life Science

355 142 ₸ Product size

100 µl 355 142 ₸ 10 µl 144 067 ₸

Order now and get it on Tuesday March 02, 2021

Anti-IDH1 antibody [EPR12296] (ab172964)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR12296] to IDH1
Suitable for: WB, IHC-P, IP
Knockout validated
Reacts with: Human

Product name

Anti-IDH1 antibody [EPR12296]
See all IDH1 primary antibodies
Description

Rabbit monoclonal [EPR12296] to IDH1
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human IDH1 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: O75874
Positive control
- WB: HAP1, HepG2, HeLa, and Raji cell lysates and human fetal kidney tissue lysate. IHC-P: Human breast carcinoma tissue. IP: HepG2 cell lysate.
General notes
This product was previously labelled as Isocitrate dehydrogenase.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR12296
Isotype

IgG
Research areas

Western blot - Anti-IDH1 antibody [EPR12296] (ab172964)

Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: IDH1 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: HepG2 whole cell lysate (20 µg)

Lanes 1 - 4: Merged signal (red and green). Green - ab172964 observed at 46 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab172964 was shown to specifically react with IDH1 in wild-type HAP1 cells. No band was observed when IDH1 knockout samples were used. Wild-type and IDH1 knockout samples were subjected to SDS-PAGE. ab172964 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-IDH1 antibody [EPR12296] (ab172964)

All lanes : Anti-IDH1 antibody [EPR12296] (ab172964) at 1/1000 dilution

Lane 1 : HepG2 lysate
Lane 2 : HeLa lysate
Lane 3 : Raji lysate
Lane 4 : Human fetal kidney lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 47 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH1 antibody [EPR12296] (ab172964)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labeling IDH1 with ab172964 at 1/100 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunoprecipitation - Anti-IDH1 antibody [EPR12296] (ab172964)

Western blot analysis on immunoprecipitation pellet from (1) HepG2 cell lysate or (2) 1X PBS (negative control) using ab172964 and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
Anti-IDH1 antibody [EPR12296] (ab172964)

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