Anti-Nkx2.5 antibody (ab272914)
Key features and details
- Rabbit polyclonal to Nkx2.5
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat
- Isotype: IgG
Overview
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Product name
Anti-Nkx2.5 antibody
See all Nkx2.5 primary antibodies -
Description
Rabbit polyclonal to Nkx2.5 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Recombinant fragment within Mouse Nkx2.5. The exact sequence is proprietary. Center region.
Database link: P42582 -
Positive control
- IHC-P: Mouse and rat heart tissue. WB: Mouse heart tissue extract.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Nkx2.5 antibody (ab272914)All lanes : Anti-Nkx2.5 antibody (ab272914) at 1/500 dilution
Lane 1 : Mouse heart tissue extract
Lane 2 : Mouse spleen tissue extract
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG7.5% SDS-PAGE
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx2.5 antibody (ab272914)Paraffin-embedded mouse heart tissue stained for Nkx2.5 using ab272914 at 1/250 dilution in immunohistochemical analysis. Antigen retrieval using citrate buffer, pH 6, 15 minutes.
Red: Beta actin.
Blue: DAPI.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx2.5 antibody (ab272914)Paraffin-embedded rat heart tissue stained for Nkx2.5 using ab272914 at 1/500 dilution in immunohistochemical analysis. Antigen retrieval using citrate buffer, pH 6, 15 minutes.
