Anti-MEF2D antibody (ab226254)
Key features and details
- Rabbit polyclonal to MEF2D
- Suitable for: IHC-P, WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-MEF2D antibody
See all MEF2D primary antibodies -
Description
Rabbit polyclonal to MEF2D -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat
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Immunogen
Synthetic peptide within Human MEF2D aa 475-521. The exact sequence is proprietary. (NP_005911.1).
Database link: Q14814 -
Positive control
- WB: HeLa, HEK-293T, Jurkat and NIH/3T3 whole cell lysates. IP: HeLa whole cell lysate. IHC-P: Human breast carcinoma and mouse renal cell carcinoma tissues.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab226254 was affinity purified using an epitope specific to MEF2D immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-MEF2D antibody (ab226254)All lanes : Anti-MEF2D antibody (ab226254) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 56 kDa
Exposure time: 30 seconds
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2D antibody (ab226254)Formalin-fixed, paraffin-embedded mouse renal cell carcinoma tissue stained for MEF2D using ab226254 at 1/200 dilution in immunohistochemical analysis.
Detection: DAB staining.
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Immunoprecipitation - Anti-MEF2D antibody (ab226254)MEF2D was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab226254 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226254 at 1 µg/ml.
Lane 1: ab226254 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 3 seconds.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2D antibody (ab226254)Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for MEF2D using ab226254 at 1/200 dilution in immunohistochemical analysis.
Detection: DAB staining. Counterstain: Hematoxylin (blue).
