Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸ 10 µl 113 913 ₸

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Anti-Calnexin antibody [EPR21240] (ab241154)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR21240] to Calnexin
Suitable for: WB, ICC/IF
Knockout validated
Reacts with: Mouse, Human

Product name

Anti-Calnexin antibody [EPR21240]
See all Calnexin primary antibodies
Description

Rabbit monoclonal [EPR21240] to Calnexin
Host species

Rabbit
Tested Applications & Species

Application Species
ICC/IF
Mouse

Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- ICC/IF: Methanol-fixed HeLa and NIH3T3 cells. WB: HAP1, NIH3T3 and MEF1 cells
General notes

This product was made using synthetic libraries and phage display technology.

This antibody is a recombinant chimeric antibody. Rabbit chimeric monoclonal antibody (Human Fab/ Rabbit Fc).

Application	Species
ICC/IF	Mouse Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA
Concentration information loading...
Purity

Immunogen affinity purified
Clonality

Monoclonal
Clone number

EPR21240
Isotype

IgG1
Research areas

Western blot - Anti-Calnexin antibody [EPR21240] (ab241154)

All lanes :

Lane 1 : HAP1 whole cell lysate
Lane 2 : HAP1 CANX KO whole cell lysate
Lane 3 : NIH3T3 whole cell lysate
Lane 4 : MEF1 whole cell lysate

Lysates/proteins at 20 µg per lane.

Performed under reducing conditions.

Predicted band size: 68 kDa
Observed band size: 80 kDa
why is the actual band size different from the predicted?

ab241154 was shown to specifically react with CANX (Calnexin) in wild type HAP1 cells. No band was observed when CANX (calnexin) knockout samples were used. Wild-type and CANX (calnexin) knockout samples were subjected to SDS-PAGE. ab241154 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1ug/ml dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
Immunocytochemistry/ Immunofluorescence - Anti-Calnexin antibody [EPR21240] (ab241154)

ab241154 staining Calnexin in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab241154 at 0.01ugml then detected with an Alexa Fluor^® 488 goat anti-rabbit secondary antibody (ab150081) at a 1/1000 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue), and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 594), at a 1/250 dilution (shown in red).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Immunocytochemistry/ Immunofluorescence - Anti-Calnexin antibody [EPR21240] (ab241154)

ab241154 staining Calnexin in NIH3T3 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab241154 at 0.5ugml then detected with an Alexa Fluor^® 488 goat anti-rabbit secondary antibody (ab150081) at a 1/1000 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue), and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 594), at a 1/250 dilution (shown in red).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Anti-Calnexin antibody [EPR21240] (ab241154)

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