2-NBDG Glucose Uptake Assay Kit (ab235976)
Key features and details
- Assay type: Cell-based (quantitative)
- Detection method: Fluorescent
- Platform: Microplate reader, Fluor. microscope, Flow cyt.
- Sample type: Suspension cells
Overview
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Product name
2-NBDG Glucose Uptake Assay Kit -
Detection method
Fluorescent -
Sample type
Suspension cells -
Assay type
Cell-based (quantitative) -
Product overview
2-NBDG Glucose Uptake Assay Kit ab235976 provides a convenient tool for studying cellular glucose uptake. The kit uses 2-NBDG, a fluorescently-labeled deoxyglucose analog, as a probe for the detection of glucose taken up by cultured cells.
The 2-NBDG assay kit is suitable for analysis with flow cytometer, fluorescent microscope or microplate reader.
2-NBDG protocol summary:
- treat cells with experimental treatment in glucose-free medium
- add 2-NBDG and incubate for 10 min
- remove cell culture supernatant and wash cells with cell-based assay buffer
- add propidium iodide at this point to exclude dead cells if analyzing with flow cytometry
- add cell-based assay buffer and analyze immediately
2-NDG can be detected with filters designed to detect FITC (Ex/Em 485/535 nm). -
Platform
Microplate reader, Fluor. microscope, Flow cyt.
Properties
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Storage instructions
Please refer to protocols. -
Components 1 x 96 tests Cell-Based Assay Apigenin 1 x 100µl Cell-Based Assay Buffer Tablet 1 tablet Cell-Based Assay NBD Glucose 1 x 500µl Cell-Based Propidium Iodide Solution 1 x 250µl
Images
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NBD-glucose is taken up by Jurkat cells in a dose-dependent manner
Jurkat (Human T cell leukemia cell line from peripheral blood) cells were equilibrated in glucose-free medium for two hours prior to being treated with the indicated dilution of NBD-glucose for 10 minutes at 37°C. Cells were washed and data were collected on a MACSQuant cytometer. NBD-glucose fluorescence within the live population is shown.