Anti-Calnexin antibody (ab265602)
Key features and details
- Rabbit polyclonal to Calnexin
- Suitable for: IHC-P, WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Calnexin antibody
See all Calnexin primary antibodies -
Description
Rabbit polyclonal to Calnexin -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Orangutan
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Immunogen
Synthetic peptide within Human Calnexin aa 1-50. The exact sequence is proprietary. NP_001737.1
Database link: P27824 -
Positive control
- IHC-P: Human breast carcinoma tissue. WB: HeLa, HEK-293T and Jurkat whole cell lysate. IP: HeLa whole cell lysate.
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General notes
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Calnexin antibody (ab265602)All lanes : Anti-Calnexin antibody (ab265602) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HEK-239T whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Predicted band size: 68 kDa
Exposure time: 3 seconds
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calnexin antibody (ab265602)Immunohistochemical analysis of formalin-fixed, paraffin-embedded human breast carcinoma tissue labeling Calnexin with ab265602 at 1/1000 dilution. Detection: DAB.
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Immunoprecipitation - Anti-Calnexin antibody (ab265602)Calnexin was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab265602 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab265602 at 1 µg/ml.
Lane 1: ab265602 IP in HeLa whole cell lysate.
Lane 2: Control IgG.Exposure time: 1 sec.
