Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-DR5 antibody [EPR22276] (ab230969)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22276] to DR5
  • Suitable for: WB, IHC-P, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-DR5 antibody [EPR22276]
    See all DR5 primary antibodies

  • Description

    Rabbit monoclonal [EPR22276] to DR5

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HepG2, HT-1080 and U-87 MG whole cell lysate. Doxorubicin (ab120629) treated HCT116 cell lysate. IHC-P: Human colon cancer and endometrium tissue. IP: DR5 IP in U-87 MG whole cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DR5 antibody [EPR22276] (ab230969)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DR5 antibody [EPR22276] (ab230969)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue labeling DR5 with ab230969 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Membranous and cytoplasmic staining in human colon cancer cells (PMID: 23284732) is observed. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DR5 antibody [EPR22276] (ab230969)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DR5 antibody [EPR22276] (ab230969)

    Immunohistochemical analysis of paraffin-embedded human endometrium tissue labeling DR5 with ab230969 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining in endothelial cells of human endometrium (PMID: 22789540, 12874246, 15987601) is observed. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Western blot - Anti-DR5 antibody [EPR22276] (ab230969)
    Western blot - Anti-DR5 antibody [EPR22276] (ab230969)
    All lanes : Anti-DR5 antibody [EPR22276] (ab230969) at 1/1000 dilution

    Lane 1 : Untreated HCT116 (human colorectal carcinoma epithelial cell), whole cell lysate
    Lane 2 : HCT116 treated with 0.5 µM doxorubicin (ab120629) for 24 hours, whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 47 kDa


    Exposure time: 3 minutes


    The molecular weight observed is consistent with what has been described in the literature, the 40kDa band is a cleaved form (PMID: 20515924, 16297203).

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-DR5 antibody [EPR22276] (ab230969)
    Western blot - Anti-DR5 antibody [EPR22276] (ab230969)
    All lanes : Anti-DR5 antibody [EPR22276] (ab230969) at 1/1000 dilution

    Lane 1 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate
    Lane 2 : HT-1080 (human fibrosarcoma epithelial cell), whole cell lysate
    Lane 3 : U-87 MG (human glioblastoma-astrocytoma epithelial cell), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 47 kDa



    The molecular weight observed is consistent with what has been described in the literature. The 40kDa band is a cleaved form (PMID: 20515924, 16297203).

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lanes 1: 3 minutes; Lanes 2-3: 59 seconds.

  • Immunoprecipitation - Anti-DR5 antibody [EPR22276] (ab230969)
    Immunoprecipitation - Anti-DR5 antibody [EPR22276] (ab230969)

    DR5 was immunoprecipitated from 0.35 mg U-87 MG (human glioblastoma-astrocytoma epithelial cell) whole cell lysate with ab230969 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab230969 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.
    Lane 1: U-87 MG whole cell lysate 10 µg (Input).
    Lane 2: ab230969 IP in U-87 MG whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab230969 in U-87 MG whole cell lysate.
    Blocking/Dilution buffer: 5% NFDM/TBST.
    Exposure time: 20 seconds.

    The molecular weight observed is consistent with what has been described in the literature. The 40kDa band is a cleaved form (PMID: 20515924, 16297203).

  • Anti-DR5 antibody [EPR22276] (ab230969)
    Anti-DR5 antibody [EPR22276] (ab230969)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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