Mitochondrial Staining Kit - Orange (Ex405nm) - Cytopainter (ab138897)
Overview
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Product name
Mitochondrial Staining Kit - Orange (Ex405nm) - Cytopainter
See all Mitochondria kits -
Detection method
Fluorescent -
Sample type
Adherent cells, Suspension cells -
Assay type
Cell-based -
Product overview
Abcam's fluorescence imaging kits are a set of fluorescence imaging tools for labeling sub-cellular organelles such as membranes, lysosomes, mitochondria, nuclei, etc. The selective labeling of live cell compartments provides a powerful method for studying cellular events in a spatial and temporal context.
ab138897 is designed to label mitochondria of live cells in orange fluorescence of large Stokes Shift at Ex/Em = 405/550 nm. The kit uses a proprietary dye that selectively accumulates in mitochondria probably via the mitochondrial membrane potential gradient. The mitochondrial indicator, a hydrophobic compound, easily permeates intact live cells and trapped in mitochondria after it gets into cells. This fluorescent mitochondrial indicator is retained in mitochondria for a long time since it carries a cell-retaining group. This key feature significantly increases the staining efficiency. The mitochondrial stain used in the kit is quite photostable, making the fluorescence quite robust. The kit provides all the essential components with an optimized cell-labeling protocol. It is suitable for proliferating and non-proliferating cells.
Review other dyes and kits for mitochondrial staining, or the live cell staining fluorescent dyes guide
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Notes
Mitochondria are membrane-enclosed organelles found in most eukaryotic cells. Mitochondria are sometimes described as “cellular power plants” because they generate most of the cellular supply of ATP. In addition to supplying cellular energy, mitochondria are involved in a range of other processes, such as signaling, cellular differentiation, cell death, as well as the control of the cell cycle and cell growth. Mitochondria have been implicated in several human diseases, including mitochondrial disorders and cardiac dysfunction, and may play a role in the aging process. Although most of a cellular DNA is contained in the cell nucleus, the mitochondrion has its own independent genome.
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Platform
Fluorescence microscope
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 500 tests Live Cell Staining Buffer 1 x 50ml MitoViolet Indicator 1 x 100µl -
Research areas
Images
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Fluorescence Microscopy of U2OS cellsImage of U2OS cells stained with ab138897 in a black 96-well plate.