Anti-Eph receptor B1 + Eph receptor B2 + Eph receptor B3 + Eph receptor B4 antibody (ab64820)
Key features and details
- Rabbit polyclonal to Eph receptor B1 + Eph receptor B2 + Eph receptor B3 + Eph receptor B4
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Eph receptor B1 + Eph receptor B2 + Eph receptor B3 + Eph receptor B4 antibody -
Description
Rabbit polyclonal to Eph receptor B1 + Eph receptor B2 + Eph receptor B3 + Eph receptor B4 -
Host species
Rabbit -
Specificity
ab64820 detects endogenous levels of total Eph receptor B4 protein. We have not performed cross-reactivity testing to other class B Eph receptors. The immunogen has high homology to EPHB1(100%), EPHB3 (100%), EPHB2 (92%). -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human Eph receptor B1 + Eph receptor B2 + Eph receptor B3 + Eph receptor B4 aa 571-610 (internal sequence).
Database link: P54760 -
Positive control
- WB: 293 cell, Jurkat, HY926 cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab64820 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Eph receptor B1 + Eph receptor B2 + Eph receptor B3 + Eph receptor B4 antibody (ab64820)All lanes : Anti-Eph receptor B1 + Eph receptor B2 + Eph receptor B3 + Eph receptor B4 antibody (ab64820) at 1/500 dilution
Lane 1 : 293 cell extract at 30 µg
Lane 2 : Jurkat cell extract at 30 µg
Lane 3 : immunising peptide at 10 µg
Predicted band size: 108 kDa
Observed band size: 108 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eph receptor B1 + Eph receptor B2 + Eph receptor B3 + Eph receptor B4 antibody (ab64820)IHC image of ab64820 staining in human normal lymphoid formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab64820, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Western blot - Anti-Eph receptor B1 + Eph receptor B2 + Eph receptor B3 + Eph receptor B4 antibody (ab64820)Anti-Eph receptor B1 + Eph receptor B2 + Eph receptor B3 + Eph receptor B4 antibody (ab64820) at 1/2000 dilution + HY926 cell lysate
Predicted band size: 108 kDa
