Ipilimumab ELISA Kit (Yervoy®) (ab237653)
Key features and details
- Sensitivity: 10 ng/ml
- Range: 10 ng/ml - 1000 ng/ml
- Sample type: Plasma, Serum
- Detection method: Colorimetric
- Assay type: Quantitative
- Reacts with: Human
Overview
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Product name
Ipilimumab ELISA Kit (Yervoy®)
See all Ipilimumab kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Serum, Plasma -
Assay type
Quantitative -
Sensitivity
10 ng/ml -
Range
10 ng/ml - 1000 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 85% - 115% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Ipilimumab ELISA Kit (Yervoy®) (ab237640) is a highly specific and sensitive kit designed for the in vitro determination of Ipilimumab in human serum and plasma. The density of color is proportional to the amount of Ipilimumab captured from the samples and can be quantified when compared with standard curve.
Ipilimumab (Yervoy®) is a fully human IgG1κ antibody that binds to CTLA-4 (cytotoxic T lymphocyte associated antigen 4), a molecule on Tcells that is indicated for unresectable or metastatic melanoma. The absence or presence of CTLA-4 can augment or suppress the immune system's T-cell response in fighting disease. Ipilimumab is designed to block the activity of CTLA-4, thereby sustaining an active immune response in its attack on cancer cells. The proposed mechanism of action is indirect, and may be through T-cell - mediated anti-tumor immune responses.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 96 tests Assay Buffer 1 x 50ml HRP-conjugate Probe 1 x 12ml Ipilimumab Standard S1 1 x 0.3ml Ipilimumab Standard S2 1 x 0.3ml Ipilimumab Standard S3 1 x 0.3ml Ipilimumab Standard S4 1 x 0.3ml Ipilimumab Standard S5 1 x 0.3ml Ipilimumab Standard S6 1 x 0.3ml Ipilimumab Standard S7 1 x 0.3ml Micro ELISA Plate 1 unit Plate sealers 2 units Stop Solution 1 x 12ml TMB substrate 1 x 12ml Wash buffer (20X) 1 x 50ml -
Relevance
Ipilimumab (Yervoy®) is a fully human IgG1? antibody that binds to CTLA-4 (cytotoxic T lymphocyteassociated antigen 4), a molecule on Tcells that is indicated for unresectable or metastatic melanoma. The absence or presence of CTLA-4 can augment or suppress the immune system's T-cell response in fighting disease. Ipilimumab is designed to block the activity of CTLA-4, thereby sustaining an active immune response in its attack on cancer cells. The proposed mechanism of action is indirect, and may be through T-cell - mediated anti-tumor immune responses. -
Alternative names
- Yervoy
Images
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Example of Ipilimumab standard curve
Typical Standard Curve: This standard curve is for demonstration only. A standard curve must be run with each assay.