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Mouse GM-CSF ELISA Kit (CSF2) (ab201276)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 3.1 pg/ml
  • Range: 12.5 pg/ml - 800 pg/ml
  • Sample type: Cell culture supernatant
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Mouse

Overview

  • Product name

    Mouse GM-CSF ELISA Kit (CSF2)
    See all GM-CSF kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 5 6%
    Inter-assay
    Sample n Mean SD CV%
    Overall 3 5.8%

  • Sample type

    Cell culture supernatant

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    3.1 pg/ml

  • Range

    12.5 pg/ml - 800 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 98 87% - 106%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Mouse

  • Product overview

    Mouse GM-CSF ELISA Kit (CSF2) (ab201276) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of GM-CSF (CSF2) protein in cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse GM-CSF (CSF2) with 3.1 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    Mouse GM-CSF (CSF-2) is a ~25 kDa pleiotropic cytokine produced by a variety of cells (activated T and B cells, macrophages, mast cells, fibroblasts, endothelial cells) during inflammation or in response to cytokines. GM-CSF can induce proliferation and differentiation of myeloid progenitor cells into eosinophils, macrophages/monocytes, and neutrophils. It is also a survival factor for mature eosinophils, macrophages/monocytes, and neutrophils.

  • Platform

    Microplate (12 x 8 well strips)

Properties

Images

  • Other - Mouse GM-CSF ELISA Kit (CSF2) (ab201276)
    Other - Mouse GM-CSF ELISA Kit (CSF2) (ab201276)

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Mouse GM-CSF (CSF2) standard curve comparison data.
    Mouse GM-CSF (CSF2) standard curve comparison data.

    Standard curve comparison between mouse GM-CSF (CSF2) SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows comparable sensitivity.

  • Example of GM-CSF standard curve.
    Example of GM-CSF standard curve.

    Background-subtracted data values (mean +/- SD) are graphed.

  • Linearity of dilution of the assay. Interpolated concentrations of GM-CSF in mouse cell culture media.
    Linearity of dilution of the assay. Interpolated concentrations of GM-CSF in mouse cell culture media.

    The concentrations of GM-CSF were measured in duplicate and interpolated from the GM-CSF standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean GM-CSF concentration was determined to be 289.7 pg/mL in mouse RAW LPS-stimulated supernatant.

  • Comparison of secreted GM-CSF in unstimulated and LPS stimulated RAW264.7 Cells.
    Comparison of secreted GM-CSF in unstimulated and LPS stimulated RAW264.7 Cells.

    RAW264.7 cells were grown in the absence (unstimulated) or presence of 5 µg/mL Lipopolysaccharide (LPS) (stimulated) for 48 hours. GM-CSF was measured in undiluted cell culture supernatants of unstimulated and LPS stimulated RAW264.7. Measured values were interpolated from the GM-CSF Standard Curve diluted in Sample Diluent NS. Mean of duplicate values +/-SD are graphed: 4.1 pg/mL unstimulated, 269.5 pg/mL stimulated.

  • Sandwich ELISA - Mouse GM-CSF ELISA Kit (CSF2) (ab201276)
    Sandwich ELISA - Mouse GM-CSF ELISA Kit (CSF2) (ab201276)
    To learn more about the advantages of recombinant antibodies see here.

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