Anti-DR5 antibody (ab8416)
Key features and details
- Rabbit polyclonal to DR5
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-DR5 antibody
See all DR5 primary antibodies -
Description
Rabbit polyclonal to DR5 -
Host species
Rabbit -
Specificity
Does not cross react with DR4. -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P MouseWB MouseHuman
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Immunogen
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Positive control
- IHC-P: Mouse kidney tissue. WB: HeLa and K562 whole cell lysate. ICC/IF: HeLa cells.
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General notes
For information on sample preparation for western blot, please refer here.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-DR5 antibody (ab8416)All lanes : Anti-DR5 antibody (ab8416) at 0.5 µg/ml
Lane 1 : HEK293 cell lysate
Lane 2 : A431 cell lysate
Lane 3 : A549 cell lysate
Lane 4 : Caco-2 cell lysate
Lane 5 : Daudi cell lysate
Lane 6 : HeLa cell lysate
Lane 7 : HepG2 cell lysate
Lane 8 : K562 cell lysate
Lane 9 : MCF7 cell lysate
Lane 10 : Jurkat cell lysate
Lane 11 : SK-N-SH cell lysate
Lane 12 : THP-1 cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 47.8 kDa
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Western blot - Anti-DR5 antibody (ab8416)All lanes : Anti-DR5 antibody (ab8416) at 1 µg/ml
Lane 1 : 3T3/NIH cell lysate
Lane 2 : C2C12 cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 47.8 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DR5 antibody (ab8416)Immunohistochemical analysis of 4% paraformaldehydefixed mouse kidney tissue using anti-DR5 antibody (ab8416) at 20 ug/mL followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DR5 antibody (ab8416)Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-DR5 antibody (ab8416) at 5 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation (microwave for 20 minutes) with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary at room temperature for 1 hour. Counter stained with Hematoxylin.
Endogenous peroxidase blocking: 3% H202 at room temperature for 10 minutes.
Washing: PBS, 2 x 5 minutes.
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Immunocytochemistry/ Immunofluorescence - Anti-DR5 antibody (ab8416)Immunofluorescent analysis of 4% paraformaldehyde-fixed (room temperature for 10 minutes) HeLa cells labeling DR5 with ab8416 at 20 ug/mL, followed by goat anti-rabbit IgG antibody (Alexa Fluor 488) at 1/500 dilution (green).
Blocking condition: 5% BSA, room temperature for 1 hour.
Primary antibody incubation: 4°C overnight.
Secondary antibody incubation: room temperature for 1 hour.
Washing condition: PBS, 3 x 3 minutes
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Immunocytochemistry/ Immunofluorescence - Anti-DR5 antibody (ab8416)ab8416 at 5µg/ml staining DR5 in Hela cells by ICC. Cells were fixed with formaldehyde and blocked with 10% serum for 1 hour at room termperature. Antigen retrieval was by heat mediation with a Citrate buffer (pH 6). Overnight primary antibody at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary.
