PE Anti-CRISPR-Cas9 antibody [EPR19799] (ab215701)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- PE Rabbit monoclonal [EPR19799] to CRISPR-Cas9
- Suitable for: Flow Cyt
- Reacts with: Staphylococcus aureus
- Conjugation: PE. Ex: 488nm, Em: 575nm
Overview
-
Product name
PE Anti-CRISPR-Cas9 antibody [EPR19799]
See all CRISPR-Cas9 primary antibodies -
Description
PE Rabbit monoclonal [EPR19799] to CRISPR-Cas9 -
Host species
Rabbit -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Staphylococcus aureus -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- Flow Cyt: 293T cells transfected with CRISPR-Cas9 with Myc tag
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19799 -
Isotype
IgG
Images
-
Flow cytometric analysis of 293T transfected with CRISPR-Cas9 with Myc tag stained with ab215701 (right panel). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 90% methanol. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab215701, 1/500 dilution) for 30 min at 22°C.
Isotype control antibody (left panel) was Rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Co-staining with anti-myc (y-axis).
Acquisition of >5,000 events were collected using a 20 mW blue solid-state laser (488nm) and 585/42 bandpass filter.
-