This kit contains protocols and reagents for use in the detection of Multiple Drug Resistance within cells.

Multiple drug resistance is characterized by over-expression of membrane transport proteins ABCG2 and ABCB1 that induce efflux of anticancer drugs out of the cell and subsequently cause tumor cell resistance to those drugs. Overcoming MDR has become a major challenge for chemotherapy. Early detection of drug resistant tumor cells may be critical in successful treatment of tumors. In addition, multiple drug resistance has become important for treatment of several other diseases such as tuberculosis, cholera, malaria and HIV-AIDS. The MarkerGeneTM Multiple Drug Resistance Microtiterplate Assay Kit is a high-throughput assay system based upon measurement of efflux of a green fluorescent dye that is known to bind to the MDR transporters ABCG2 and ABCB1 in a manner similar to many chemotherapeutic drugs. The efflux of this dye can be easily measured by its fluorescence in the media (EM 504nm, EX 538nm).

This kit is sold by Marker Gene under product code M1580.