HRP Anti-ATG7 antibody [EPR6251] (ab215336)
![HRP Anti-ATG7 antibody [EPR6251] (ab215336)](https://www.abcam.com/ps/products/215/ab215336/Images/ab215336-310897-ab215336KOWBJRIZ.jpg "HRP Anti-ATG7 antibody [EPR6251] (ab215336)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR6251] to ATG7
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Conjugation: HRP
Overview
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Product name
HRP Anti-ATG7 antibody [EPR6251]
See all ATG7 primary antibodies -
Description
HRP Rabbit monoclonal [EPR6251] to ATG7 -
Host species
Rabbit -
Conjugation
HRP -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HepG2, Hek293, Jurkat, HAP1 WT (shows pos.), Apg7 KO HAP1 (shows neg.)
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 1% BSA, 30% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6251 -
Isotype
IgG -
Research areas
Images
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Western blot - HRP Anti-ATG7 antibody [EPR6251] (ab215336)All lanes : HRP Anti-ATG7 antibody [EPR6251] (ab215336) at 1/5000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : ATG7 knockout HAP1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 77 kDa
Exposure time: 20 minutesab215336 was shown to specifically react with Apg7 in wild-type HAP1 cells as signal was lost in ATG7 knockout cells. Wild-type and ATG7 knockout samples were subjected to SDS-PAGE. Ab215336 and ab184095 (Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control (Alexa Fluor® 680) loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. The loading control was imaged using the Licor Odyssey CLx prior to blots being developed with ECL technique.
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![Western blot - HRP Anti-ATG7 antibody [EPR6251] (ab215336)](https://www.abcam.com/ps/products/215/ab215336/Images/ab215336-284044-WBab2153361.jpg)
Western blot - HRP Anti-ATG7 antibody [EPR6251] (ab215336)All lanes : HRP Anti-ATG7 antibody [EPR6251] (ab215336) at 1/5000 dilution
Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 10 µg
Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg
Lane 4 : HAP1 WT at 20 µg
Lane 5 : Apg7 KO HAP1 at 20 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 77 kDa
Observed band size: 77 kDa
Exposure time: 20 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab215336 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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![HRP Anti-ATG7 antibody [EPR6251] (ab215336)](https://www.abcam.com/ps/products/215/ab215336/Images/ab215336-3-benefits-of-recombinant-antibodies.png)
HRP Anti-ATG7 antibody [EPR6251] (ab215336)
