Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-DPF2/REQ antibody [EPR9206(B)] - BSA and Azide free (ab232327)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9206(B)] to DPF2/REQ - BSA and Azide free
  • Suitable for: WB, IP, IHC-P, ICC/IF
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-DPF2/REQ antibody [EPR9206(B)] - BSA and Azide free
    See all DPF2/REQ primary antibodies

  • Description

    Rabbit monoclonal [EPR9206(B)] to DPF2/REQ - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: A431, HeLa and LNCaP cell lysates. IHC-P: Human breast carcinoma, kidney and colon tissues; Rat and mouse cerebral cortex tissues. IP: HeLa cell lysate. ICC: HeLa cells.

  • General notes

    ab232327 is the carrier-free version of ab134942 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab232327 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as DPF2

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-DPF2/REQ antibody [EPR9206(B)] - BSA and Azide free (ab232327)
    Western blot - Anti-DPF2/REQ antibody [EPR9206(B)] - BSA and Azide free (ab232327)
    All lanes : Anti-DPF2/REQ antibody [EPR9206(B)] (ab134942) at 1/1000 dilution

    Lane 1 : Wild-type A431 cell lysate
    Lane 2 : DPF2 knockout A431 cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : LNCaP cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 44 kDa
    Observed band size: 50 kDa
    why is the actual band size different from the predicted?



    This data was developed using the same antibody clone in a different buffer formulation (ab134942).

    Lanes 1 - 4: Merged signal (red and green). Green - ab134942 observed at 50 kDa. Red - loading control, ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55kDa.

    ab134942 was shown to react with DPF2/REQ in wild-type A431 cells in western blot. Loss of signal was observed when DPF2 knockout sample was used. Wild-type and DPF2 knockout A431 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab134942 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4°C at a 1 in 1000 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunoprecipitation - Anti-DPF2/REQ antibody [EPR9206(B)] - BSA and Azide free (ab232327)
    Immunoprecipitation - Anti-DPF2/REQ antibody [EPR9206(B)] - BSA and Azide free (ab232327)

    ab134942 (purified) at 1/100 dilution (2µg) immunoprecipitating DPF2/REQ in HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate.

    Lane 1 (input): HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate 10ug
    Lane 2 (+): ab134942 + HeLa whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab134942 in HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate

    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134942).

  • Immunocytochemistry/ Immunofluorescence - Anti-DPF2/REQ antibody [EPR9206(B)] - BSA and Azide free (ab232327)
    Immunocytochemistry/ Immunofluorescence - Anti-DPF2/REQ antibody [EPR9206(B)] - BSA and Azide free (ab232327)

    Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling DPF2/REQ with purified ab134942 at 1/200 dilution (8.3μg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889, an anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 (2.5 μg/ml). ab150077, a Goat anti-rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1/1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134942).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPF2/REQ antibody [EPR9206(B)] - BSA and Azide free (ab232327)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPF2/REQ antibody [EPR9206(B)] - BSA and Azide free (ab232327)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat cerebral cortex tissue sections labeling DPF2/REQ with purified ab134942 at 1/200 dilution (8.3 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, pH9. Tissue was counterstained with hematoxylin. ab97051, a Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1/500 dilution. PBS instead of the primary antibody was used as the negative control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134942).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPF2/REQ antibody [EPR9206(B)] - BSA and Azide free (ab232327)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPF2/REQ antibody [EPR9206(B)] - BSA and Azide free (ab232327)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cerebral cortex tissue sections labeling DPF2/REQ with purified ab134942 at 1/200 dilution (8.3 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, pH9. Tissue was counterstained with hematoxylin. ab97051, a Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1/500 dilution. PBS instead of the primary antibody was used as the negative control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134942).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPF2/REQ antibody [EPR9206(B)] - BSA and Azide free (ab232327)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPF2/REQ antibody [EPR9206(B)] - BSA and Azide free (ab232327)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue sections labeling DPF2/REQ with purified ab134942 at 1/200 dilution (8.3 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, pH9. Tissue was counterstained with hematoxylin. ab97051, a Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1/500 dilution. PBS instead of the primary antibody was used as the negative control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134942).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPF2/REQ antibody [EPR9206(B)] - BSA and Azide free (ab232327)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPF2/REQ antibody [EPR9206(B)] - BSA and Azide free (ab232327)

    Immunohistochemical analysis of paraffin-embedded, formalin-fixed Human kidney tissue, labelling DPF2/REQ using unpurified ab134942 at a 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134942).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-DPF2/REQ antibody [EPR9206(B)] - BSA and Azide free (ab232327)
    OI-RD Scanning - Anti-DPF2/REQ antibody [EPR9206(B)] - BSA and Azide free (ab232327)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134942).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPF2/REQ antibody [EPR9206(B)] - BSA and Azide free (ab232327)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPF2/REQ antibody [EPR9206(B)] - BSA and Azide free (ab232327)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue sections labeling DPF2/REQ with purified ab134942 at 1/200 dilution (8.3 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, pH9. Tissue was counterstained with hematoxylin. ab97051, a Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1/500 dilution. PBS instead of the primary antibody was used as the negative control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134942).

  • Anti-DPF2/REQ antibody [EPR9206(B)] - BSA and Azide free (ab232327)
    Anti-DPF2/REQ antibody [EPR9206(B)] - BSA and Azide free (ab232327)

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