FITC Anti-Glycophorin A + B antibody [HIR2] (ab52452)
![FITC Anti-Glycophorin A + B antibody [HIR2] (ab52452)](https://www.abcam.com/ps/products/52/ab52452/Images/ab52452-1-fitc-anti-glycophorin-a-b-antibody-hir2-flow-cytometry-peripheral-blood-human.jpg "FITC Anti-Glycophorin A + B antibody [HIR2] (ab52452)")
Key features and details
- FITC Mouse monoclonal [HIR2] to Glycophorin A + B
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG2b
Overview
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Product name
FITC Anti-Glycophorin A + B antibody [HIR2]
See all Glycophorin A + B primary antibodies -
Description
FITC Mouse monoclonal [HIR2] to Glycophorin A + B -
Host species
Mouse -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Specificity
ab52452 recognises N-terminal, homologous portion of glycophorins A (GPA) and B (GPB) which are single-pass membrane sialoglycoproteins expressed on red blood cells and erythroid precursor cells. -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt Human
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Immunogen
Synthetic peptide corresponding to Human Glycophorin A + B (N terminal).
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Positive control
- Flow Cyt: A subset of erythrocytes in human peripheral blood.
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General notes
ab52452 agglutinates untreated RBCs but it failes to agglutinate papain-treated cells, binds to GPA, but weakly to GPB. The antibody is useful in erythroid cell development studies, because HIR2 antigen is expressed on early erythroblasts, late erythroblasts, erythroblasts, mature erythrocytes and the cells of erythroid cell lines K562 and HEL, but not on all other cells (mature erythrocytes are characteristically CD235a positive and CD45 and CD71 negative).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: 0.2% BSA, PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Prior to conjugation, ab52452 was purified by Protein G affinity chromatography. After conjugation, it was purified by site exclusion chromatography. Purity >95% by SDS-PAGE. -
Primary antibody notes
ab52452 agglutinates untreated RBCs but it failes to agglutinate papain-treated cells, binds to GPA, but weakly to GPB. The antibody is useful in erythroid cell development studies, because HIR2 antigen is expressed on early erythroblasts, late erythroblasts, erythroblasts, mature erythrocytes and the cells of erythroid cell lines K562 and HEL, but not on all other cells (mature erythrocytes are characteristically CD235a positive and CD45 and CD71 negative). -
Clonality
Monoclonal -
Clone number
HIR2 -
Isotype
IgG2b -
Research areas
Images
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Flow Cytometry - FITC Anti-Glycophorin A + B antibody [HIR2] (ab52452)Flow cytometry analysis (surface staining) of human peripheral blood labeling CD235a with ab52452.
