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FITC Anti-Glycophorin A + B antibody [HIR2] (ab52452)

Key features and details

  • FITC Mouse monoclonal [HIR2] to Glycophorin A + B
  • Suitable for: Flow Cyt
  • Reacts with: Human
  • Conjugation: FITC. Ex: 493nm, Em: 528nm
  • Isotype: IgG2b

Overview

  • Product name

    FITC Anti-Glycophorin A + B antibody [HIR2]
    See all Glycophorin A + B primary antibodies

  • Description

    FITC Mouse monoclonal [HIR2] to Glycophorin A + B

  • Host species

    Mouse

  • Conjugation

    FITC. Ex: 493nm, Em: 528nm

  • Specificity

    ab52452 recognises N-terminal, homologous portion of glycophorins A (GPA) and B (GPB) which are single-pass membrane sialoglycoproteins expressed on red blood cells and erythroid precursor cells.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to Human Glycophorin A + B (N terminal).

  • Positive control

    • Flow Cyt: A subset of erythrocytes in human peripheral blood.

  • General notes

    ab52452 agglutinates untreated RBCs but it failes to agglutinate papain-treated cells, binds to GPA, but weakly to GPB. The antibody is useful in erythroid cell development studies, because HIR2 antigen is expressed on early erythroblasts, late erythroblasts, erythroblasts, mature erythrocytes and the cells of erythroid cell lines K562 and HEL, but not on all other cells (mature erythrocytes are characteristically CD235a positive and CD45 and CD71 negative).

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    pH: 7.4
    Preservative: 0.097% Sodium azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...

  • Purity

    Protein G purified

  • Purification notes

    Prior to conjugation, ab52452 was purified by Protein G affinity chromatography. After conjugation, it was purified by site exclusion chromatography. Purity >95% by SDS-PAGE.

  • Primary antibody notes

    ab52452 agglutinates untreated RBCs but it failes to agglutinate papain-treated cells, binds to GPA, but weakly to GPB. The antibody is useful in erythroid cell development studies, because HIR2 antigen is expressed on early erythroblasts, late erythroblasts, erythroblasts, mature erythrocytes and the cells of erythroid cell lines K562 and HEL, but not on all other cells (mature erythrocytes are characteristically CD235a positive and CD45 and CD71 negative).

  • Clonality

    Monoclonal

  • Clone number

    HIR2

  • Isotype

    IgG2b

  • Research areas

Images

  • Flow Cytometry - FITC Anti-Glycophorin A + B antibody [HIR2] (ab52452)
    Flow Cytometry - FITC Anti-Glycophorin A + B antibody [HIR2] (ab52452)
    Flow cytometry analysis (surface staining) of human peripheral blood labeling CD235a with ab52452.

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