Anti-Blooms Syndrome Protein Blm (phospho T99) antibody (ab62206)
Key features and details
- Rabbit polyclonal to Blooms Syndrome Protein Blm (phospho T99)
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-Blooms Syndrome Protein Blm (phospho T99) antibody
See all Blooms Syndrome Protein Blm primary antibodies -
Description
Rabbit polyclonal to Blooms Syndrome Protein Blm (phospho T99) -
Host species
Rabbit -
Specificity
ab62206 detects endogenous levels of Bloom Syndrome Protein Blm only when phosphorylated at threonine 99. -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic phosphopeptide derived from human Bloom Syndrome Protein Blm around the phosphorylation site of threonine 99 (Q-E-TP-Q-R).
-
Positive control
- HeLa,HepG2 cells
-
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab62206 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-Blooms Syndrome Protein Blm (phospho T99) antibody (ab62206)All lanes : Anti-Blooms Syndrome Protein Blm (phospho T99) antibody (ab62206)
Lane 1 : HepG2 cell lysate
Lane 2 : HepG2 cell lysate with blocking peptide
Predicted band size: 159 kDa
Observed band size: 159 kDa
-
Immunocytochemistry/ Immunofluorescence - Anti-Blooms Syndrome Protein Blm (phospho T99) antibody (ab62206)Immunofluorescence analysis of HeLa cells using Blooms Syndrome Protein Blm (phospho T99) antibody (ab62206) at 1/500 dilution, in the presence (right) or absence (left) of immunising phosphopeptide. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Blooms Syndrome Protein Blm (phospho T99) antibody (ab62206)ab62206 staining Blooms Syndrome Protein Blm (phospho T99) in Human heart by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). The picture on the right is treated with the synthesized peptide.
