Antibodies, Proteins, Kits and Reagents for Life Science

288 134 ₸ Product size

100 µl 288 134 ₸

Order now and get it on Tuesday March 09, 2021

Anti-NUMB antibody [EPR21988] (ab220362)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR21988] to NUMB
Suitable for: WB, IHC-P, IP, Flow Cyt
Reacts with: Mouse, Rat, Human

Product name

Anti-NUMB antibody [EPR21988]
See all NUMB primary antibodies
Description

Rabbit monoclonal [EPR21988] to NUMB
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-P	Mouse Rat Human
IP	Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HeLa, Ramos, SK-OV-3, PC-12 and NIH/3T3 cell lysate; Mouse brain and lung lysate; Rat brain lysate. IHC-P: Human breast and kidney tissue; Mouse and rat kidney tissue. Flow cytometry: Ramos cells. IP: HeLa whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR21988
Isotype

IgG
Research areas

Western blot - Anti-NUMB antibody [EPR21988] (ab220362)

All lanes : Anti-NUMB antibody [EPR21988] (ab220362) at 1/1000 dilution

Lane 1 : Mouse brain lysate
Lane 2 : Rat brain lysate
Lane 3 : Mouse lung lysate
Lane 4 : PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate
Lane 5 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 71 kDa

Blocking/diluting buffer and concentration: 5% NFDM/TBST

Exposure time: Line 1-2: 3 minutes; Line 3: 93 seconds; Line 4-5: 3 minutes.
Flow Cytometry - Anti-NUMB antibody [EPR21988] (ab220362)

Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized Ramos (Human Burkitt's lymphoma B lymphocyte) cell line labeling NUMB with ab220362 at 1/600 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells incubated with secondary antibody only) (blue). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.
Immunoprecipitation - Anti-NUMB antibody [EPR21988] (ab220362)

NUMB was immunoprecipitated from 0.35 mg HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate with ab220362 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab220362 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/5000 dilution.
Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10 μg(Input).
Lane 2: ab220362 IP in HeLa whole cell lysate (+).
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab220362 in HeLa whole cell lysate (-).

Blocking/Dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUMB antibody [EPR21988] (ab220362)

Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling NUMB with ab220362 at 1/1000 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rar kidney tubules (PMID:27016419) is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Perform heat-mediated antigen retrieval using Universal HIER antigen retrieval reagents (10X)(ab208572).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUMB antibody [EPR21988] (ab220362)

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling NUMB with ab220362 at 1/1000 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse kidney tubules (PMID:27016419) is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Perform heat-mediated antigen retrieval using Universal HIER antigen retrieval reagents (10X)(ab208572).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUMB antibody [EPR21988] (ab220362)

Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling NUMB with ab220362 at 1/1000 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human kidney tubules (PMID:27016419) is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Perform heat-mediated antigen retrieval using Universal HIER antigen retrieval reagents (10X)(ab208572).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUMB antibody [EPR21988] (ab220362)

Immunohistochemical analysis of paraffin-embedded human breast tissue labeling NUMB with ab220362 at 1/1000 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human breast tissue (PMID: 15492044) is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Perform heat-mediated antigen retrieval using Universal HIER antigen retrieval reagents (10X)(ab208572).
Western blot - Anti-NUMB antibody [EPR21988] (ab220362)

All lanes : Anti-NUMB antibody [EPR21988] (ab220362) at 1/1000 dilution

Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : SK-BR-3 (human breast adenocarcinoma epithelial cell) whole cell lysate
Lane 3 : T-47D (human ductal breast epithelial tumor epithelial cell) whole cell lysate
Lane 4 : Ramos (human Burkitt's lymphoma B lymphocyte) whole cell lysate
Lane 5 : SK-OV-3 (human ovarian cancer epithelial cell) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 71 kDa

Blocking/diluting buffer and concentration: 5% NFDM/TBST.

Exposure time: Line 1-3: 3 minutes; Line 4: 15 seconds; Line 5: 37 seconds.

Negative controls: SK-BR-3, T-47D (PMID: 20443831).
Anti-NUMB antibody [EPR21988] (ab220362)

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