Mouse NUMB Matched Antibody Pair Kit (ab217617)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Mouse
- Range: 62.5 pg/ml - 4000 pg/ml
Overview
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Product name
Mouse NUMB Matched Antibody Pair Kit
See all NUMB kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
19.54 pg/ml -
Range
62.5 pg/ml - 4000 pg/ml -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse NUMB Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse NUMB.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab207614. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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Platform
Reagents
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Mouse NUMB Capture Antibody 2 x 50µg 1 x 50µg Mouse NUMB Detector Antibody 2 x 12.5µg 1 x 12.5µg Mouse NUMB Lyophilized Protein 2 vials 1 vial -
Research areas
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Function
Plays a role in the process of neurogenesis. Required throughout embryonic neurogenesis to maintain neural progenitor cells, also called radial glial cells (RGCs), by allowing their daughter cells to choose progenitor over neuronal cell fate. Not required for the proliferation of neural progenitor cells before the onset of neurogenesis. Also involved postnatally in the subventricular zone (SVZ) neurogenesis by regulating SVZ neuroblasts survival and ependymal wall integrity. May also mediate local repair of brain ventricular wall damage. -
Sequence similarities
Contains 1 PID domain. -
Post-translational
modificationsIsoform 1 and isoform 2 are ubiquitinated by LNX leading to their subsequent proteasomal degradation (By similarity). Ubiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- c14_5527
- C14orf41
- FLJ31314
see all -
Database links
- Entrez Gene: 18222 Mouse
- SwissProt: Q9QZS3 Mouse
- Unigene: 4390 Mouse
Images
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Mouse NUMB standard curve.
Standard calibration curve. Background subtracted values are graphed.
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Sandwich ELISA - Mouse NUMB Matched Antibody Pair Kit (ab217617)To learn more about the advantages of recombinant antibodies see here.
