Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-USP10 antibody [EPR4261] (ab109219)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4261] to USP10
  • Suitable for: WB, IP, IHC-P, ICC/IF
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-USP10 antibody [EPR4261]
    See all USP10 primary antibodies

  • Description

    Rabbit monoclonal [EPR4261] to USP10

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, 293T, A375 and A549 cell lysates IHC-P: Human colon tissue This antibody gave a positive result when used in the following methanol fixed cell lines: MCF-7

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-USP10 antibody [EPR4261] (ab109219)
    Western blot - Anti-USP10 antibody [EPR4261] (ab109219)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: USP10 knockout HAP1 cell lysate (20 µg)
    Lane 3: A549 cell lysate (20 µg)
    Lane 4: A375 cell lysate (20 µg)
    Lanes 1 to 4: Merged signal (red and green). Green - ab109219 observed at 115 kDa. Red - loading control, ab8245, observed at 37 kDa.
    ab109219 was shown to recognize USP10 when USP10 knockout samples were used, along with additional cross-reactive bands. Wild-type and USP10 knockout samples were subjected to SDS-PAGE. ab109219 and ab8245 (loading control to GAPDH) were both diluted at 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

  • Western blot - Anti-USP10 antibody [EPR4261] (ab109219)
    Western blot - Anti-USP10 antibody [EPR4261] (ab109219)
    All lanes : Anti-USP10 antibody [EPR4261] (ab109219) at 1/1000 dilution

    Lane 1 : Hela cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : A375 cell lysate
    Lane 4 : A549 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 87 kDa
    Observed band size: 110 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP10 antibody [EPR4261] (ab109219)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP10 antibody [EPR4261] (ab109219)

    ab109219, at a 1/250 dilution, staining USP10 in paraffin embedded Human colon tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-USP10 antibody [EPR4261] (ab109219)
    Immunocytochemistry/ Immunofluorescence - Anti-USP10 antibody [EPR4261] (ab109219)

    ICC/IF image of ab109219 stained MCF-7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab109219 at 1/50 dilution overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • Anti-USP10 antibody [EPR4261] (ab109219)
    Anti-USP10 antibody [EPR4261] (ab109219)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-USP10 antibody [EPR4261] (ab109219)

© 2021 Astana Biomed Group LLP. All rights reserved.