PFA fixed, 0.15% Triton permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Notch1 using ab245686 at 10 µg/ml for 1 hour (green) followed by Alexa Fluor^® 488 secondary antibody (1 µg/ml), in ICC/IF. The nuclear counter stain is DAPI (blue).

Flow Cytometry using ab245686.

Mouse thymocytes were stained with unimmunized rabbit IgG antibody (black line) or ab245686 (blue line) at a concentration of 10 µg/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody at 2 µg/ml