Anti-NUMBL antibody (ab37500)
Key features and details
- Rabbit polyclonal to NUMBL
- Suitable for: WB, IHC-FoFr
- Reacts with: Mouse, Rat
- Isotype: IgG
Overview
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Product name
Anti-NUMBL antibody -
Description
Rabbit polyclonal to NUMBL -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-FoFrmore details -
Species reactivity
Reacts with: Mouse, Rat
Predicted to work with: Cow, Human
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Immunogen
Synthetic peptide corresponding to Human NUMBL aa 200-300 conjugated to keyhole limpet haemocyanin.
(Peptide available asab37499)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab37500 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa). IHC-FoFr 1/1000. Target
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Function
Plays a role in the process of neurogenesis. Required throughout embryonic neurogenesis to maintain neural progenitor cells, also called radial glial cells (RGCs), by allowing their daughter cells to choose progenitor over neuronal cell fate. Not required for the proliferation of neural progenitor cells before the onset of embryonic neurogenesis. Also required postnatally in the subventricular zone (SVZ) neurogenesis by regulating SVZ neuroblasts survival and ependymal wall integrity. Negative regulator of NF-kappa-B signaling pathway. The inhibition of NF-kappa-B activation is mediated at least in part, by preventing MAP3K7IP2 to interact with polyubiquitin chains of TRAF6 and RIPK1 and by stimulating the 'Lys-48'-linked polyubiquitination and degradation of TRAF6 in cortical neurons. -
Sequence similarities
Contains 1 PID domain. -
Domain
The PTB domain is necessary for the inhibition of MAP3K7IP2-mediated activation of NF-kappa-B. -
Cellular localization
Cytoplasm. Symmetrically distributed throughout the cytoplasm in non dividing neuroblasts of the CNS. - Information by UniProt
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Database links
- Entrez Gene: 9253 Human
- Entrez Gene: 18223 Mouse
- Entrez Gene: 292732 Rat
- Omim: 604018 Human
- SwissProt: Q9Y6R0 Human
- SwissProt: O08919 Mouse
- SwissProt: A1L1I3 Rat
- Unigene: 326953 Human
see all -
Alternative names
- CAG 3A antibody
- CAG3A antibody
- CTG 3a antibody
see all
Images
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Western blot - Anti-NUMBL antibody (ab37500)All lanes : Anti-NUMBL antibody (ab37500) at 1 µg/ml
Lane 1 : Brain (Mouse) Whole Cell Lysate - normal tissue
Lane 2 : Testis (Mouse) Whole Cell Lysate - normal tissue
Lane 3 :Mouse pancreas tissue lysate - total protein (ab29363)
Lane 4 : Lung (Mouse) Whole Cell Lysate - normal tissue
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 65 kDa
Observed band size: 65 kDa
Additional bands at: 70 kDa. We are unsure as to the identity of these extra bands. -
Western blot - Anti-NUMBL antibody (ab37500)All lanes : Anti-NUMBL antibody (ab37500) at 1 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Spinal Cord (Mouse) Tissue Lysate
Lane 3 : Brain (Rat) Tissue Lysate - normal tissue
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 65 kDa
Observed band size: 65-70 kDa why is the actual band size different from the predicted?
Additional bands at: 60 kDa. We are unsure as to the identity of these extra bands. -
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-NUMBL antibody (ab37500)ab37500 staining NUMBL in rat brain tissue section by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue from 4% PFA perfused animals underwent overnight fixation in 4% paraformaldehyde, cryoprotected in 30% sucrose and cut using cryostat.The primary antibody was diluted, 1/1000 (PBS + 0.3% Triton X100) and incubated with sample for 18 hours at 20°C. An Alexa Fluor® 488 conjugated goat polyclonal to rabbit IgG was used undiluted as secondary.
Protocols
Datasheets and documents
References (6)
ab37500 has been referenced in 6 publications.
- Huang Y et al. Elucidating the expression and function of Numbl during cell adhesion-mediated drug resistance (CAM-DR) in multiple myeloma (MM). BMC Cancer 19:1269 (2019). PubMed: 31888545
- García-Heredia JM & Carnero A NUMB and NUMBL differences in gene regulation. Oncotarget 9:9219-9234 (2018). PubMed: 29507685
- García-Heredia JM et al. Numb-like (NumbL) downregulation increases tumorigenicity, cancer stem cell-like properties and resistance to chemotherapy. Oncotarget 7:63611-63628 (2016). PubMed: 27613838
- Tao T et al. Numbl inhibits glioma cell migration and invasion by suppressing TRAF5-mediated NF-?B activation. Mol Biol Cell 23:2635-44 (2012). WB, ICC/IF ; Human . PubMed: 22593207
- Liu C et al. Epigenetic regulation of miR-184 by MBD1 governs neural stem cell proliferation and differentiation. Cell Stem Cell 6:433-44 (2010). WB ; Mouse . PubMed: 20452318
- Zhou L et al. NUMBL interacts with TRAF6 and promotes the degradation of TRAF6. Biochem Biophys Res Commun 392:409-14 (2010). WB, ICC/IF ; Rat . PubMed: 20079715
Images
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Western blot - Anti-NUMBL antibody (ab37500)All lanes : Anti-NUMBL antibody (ab37500) at 1 µg/ml
Lane 1 : Brain (Mouse) Whole Cell Lysate - normal tissue
Lane 2 : Testis (Mouse) Whole Cell Lysate - normal tissue
Lane 3 :Mouse pancreas tissue lysate - total protein (ab29363)
Lane 4 : Lung (Mouse) Whole Cell Lysate - normal tissue
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 65 kDa
Observed band size: 65 kDa
Additional bands at: 70 kDa. We are unsure as to the identity of these extra bands. -
Western blot - Anti-NUMBL antibody (ab37500)All lanes : Anti-NUMBL antibody (ab37500) at 1 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Spinal Cord (Mouse) Tissue Lysate
Lane 3 : Brain (Rat) Tissue Lysate - normal tissue
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 65 kDa
Observed band size: 65-70 kDa why is the actual band size different from the predicted?
Additional bands at: 60 kDa. We are unsure as to the identity of these extra bands.
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Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-NUMBL antibody (ab37500)
ab37500 staining NUMBL in rat brain tissue section by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue from 4% PFA perfused animals underwent overnight fixation in 4% paraformaldehyde, cryoprotected in 30% sucrose and cut using cryostat.The primary antibody was diluted, 1/1000 (PBS + 0.3% Triton X100) and incubated with sample for 18 hours at 20°C. An Alexa Fluor® 488 conjugated goat polyclonal to rabbit IgG was used undiluted as secondary.
