Anti-Notch1 antibody [A6] (ab44986)
![Anti-Notch1 antibody [A6] (ab44986)](https://www.abcam.com/ps/products/44/ab44986/Images/ab44986-57124-anti-notch1-antibody-a6-immunofluorescence.jpg "Anti-Notch1 antibody [A6] (ab44986)")
Key features and details
- Mouse monoclonal [A6] to Notch1
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG2b
Overview
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Product name
Anti-Notch1 antibody [A6]
See all Notch1 primary antibodies -
Description
Mouse monoclonal [A6] to Notch1 -
Host species
Mouse -
Specificity
This antibody is specific for Notch1. -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human Notch1.
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Epitope
Ligand binding region -
Positive control
- Pancreas
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: 0.01% PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
A6 -
Isotype
IgG2b -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Notch1 antibody [A6] (ab44986)ICC/IF image of ab44986 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab44986, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
