Anti-NUMB antibody (ab264224)
Key features and details
- Rabbit polyclonal to NUMB
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-NUMB antibody
See all NUMB primary antibodies -
Description
Rabbit polyclonal to NUMB -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide within Human NUMB aa 375-425. The exact sequence is proprietary.
Database link: P49757 -
Positive control
- WB: HeLa and HEK-293T whole cell lysate, IP: HeLa cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-NUMB antibody (ab264224)
NUMB was immunoprecipitated from 1 mg HeLa whole cell lysate using ab264224 at 3 µg/mg lysate. Western blot was performed on the immunoprecipitate using ab264224 at 0.1 µg/ml.
Lane 1: ab264224 IP in HeLa whole cell lysate.
Lane 2: Control IgG. -
Western blot - Anti-NUMB antibody (ab264224)All lanes : Anti-NUMB antibody (ab264224) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : HEK-293T whole cell lysate at 50 µg
Developed using the ECL technique.
Exposure time: 30 seconds
