Human Presenilin 1 ELISA Kit (ab267605)
Key features and details
- Sensitivity: 10.5 pg/ml
- Range: 10.24 pg/ml - 2500 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Presenilin 1 ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
10.5 pg/ml -
Range
10.24 pg/ml - 2500 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 77.21 69% - 89% Plasma 75.47 71% - 82% Cell culture media 70.85 67% - 76% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human Presenilin 1 ELISA Kit is designed for the quantitative determination of Presenilin 1 in cell culture supernatants, plasma and serum samples.
Δ Note: Human Presenilin 1 concentration is low in normal serum/plasma, it may not be detected in this assay.
This assay employs an antibody specific for human Presenilin 1 coated on a 96-well plate. Standards and samples are pipetted into the wells and Presenilin 1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human Presenilin 1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Presenilin 1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
This ELISA kit shows no cross-reactivity with the following cytokines tested:
Human B7-2 (CD86); BAFF R; Calcitonin; Calsyntenin-1; Cathepsin E; cIAP-2 (HIAP-1); Coagulation Factor VII; Complement MASP3; Endocan; EphA2; EphB4; Ephrin-A4; FGF-23; FGF-5; Flt-3 (Flk-2); GLP-1 (7-37 & 7-36-NH2); Glypican 2; GM-CSF R alpha; GP73 (GOLM1); HTRA2 (Omi); IL-20 R alpha; IL-4 R alpha; JAM-C; LH; Matrilin-3; Meprin alpha (MEP1A); MSP R (Ron); N-Cadherin; Neprilysin-2 (MMEL1); NKp44; PAPP-A; PTH; Pepsinogen II (PGC); PYY; SOX2; TFPI-2; TRACP (ACP5);TFF3; Ubiquitin+1.
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Platform
Microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 500X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml 5X Assay Diluent D 1 x 15ml Anti-Human Presenilin 1 coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Human Presenilin 1 Detection Antibody 2 vials Human Presenilin 1 Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Function
Probable catalytic subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein). Requires the other members of the gamma-secretase complex to have a protease activity. May play a role in intracellular signaling and gene expression or in linking chromatin to the nuclear membrane. Stimulates cell-cell adhesion though its association with the E-cadherin/catenin complex. Under conditions of apoptosis or calcium influx, cleaves E-cadherin promoting the disassembly of the E-cadherin/catenin complex and increasing the pool of cytoplasmic beta-catenin, thus negatively regulating Wnt signaling. May also play a role in hematopoiesis. -
Tissue specificity
Expressed in a wide range of tissues including various regions of the brain, liver, spleen and lymph nodes. -
Involvement in disease
Defects in PSEN1 are a cause of Alzheimer disease type 3 (AD3) [MIM:607822]. AD3 is a familial early-onset form of Alzheimer disease. Alzheimer disease is a neurodegenerative disorder characterized by progressive dementia, loss of cognitive abilities, and deposition of fibrillar amyloid proteins as intraneuronal neurofibrillary tangles, extracellular amyloid plaques and vascular amyloid deposits. The major constituent of these plaques is the neurotoxic amyloid-beta-APP 40-42 peptide (s), derived proteolytically from the transmembrane precursor protein APP by sequential secretase processing. The cytotoxic C-terminal fragments (CTFs) and the caspase-cleaved products such as C31 derived from APP, are also implicated in neuronal death.
Defects in PSEN1 are a cause of frontotemporal dementia [MIM:600274].
Defects in PSEN1 are the cause of cardiomyopathy dilated type 1U (CMD1U) [MIM:613694]. It is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
Defects in PSEN1 are the cause of acne inversa familial type 3 (ACNIF3) [MIM:613737]. A chronic relapsing inflammatory disease of the hair follicles characterized by recurrent draining sinuses, painful skin abscesses, and disfiguring scars. Manifestations typically appear after puberty. -
Sequence similarities
Belongs to the peptidase A22A family. -
Domain
The PAL motif is required for normal active site conformation. -
Post-translational
modificationsHeterogeneous proteolytic processing generates N-terminal (NTF) and C-terminal (CTF) fragments of approximately 35 and 20 kDa, respectively. During apoptosis, the C-terminal fragment (CTF) is further cleaved by caspase-3 to produce the fragment, PS1-CTF12.
After endoproteolysis, the C-terminal fragment (CTF) is phosphorylated on serine residues by PKA and/or PKC. Phosphorylation on Ser-346 inhibits endoproteolysis. -
Cellular localization
Endoplasmic reticulum membrane. Golgi apparatus membrane. Cell surface. Bound to NOTCH1 also at the cell surface. Colocalizes with CDH1/2 at sites of cell-cell contact. Colocalizes with CTNNB1 in the endoplasmic reticulum and the proximity of the plasma membrane. Also present in azurophil granules of neutrophils. - Information by UniProt
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Alternative names
- AD3
- Ad3h
- FAD
see all -
Database links
- Entrez Gene: 5663 Human
- Omim: 104311 Human
- SwissProt: P49768 Human
- Unigene: 3260 Human
Images
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Example data
This standard curve is for demonstration only. A standard curve must be run with each assay.