Antibodies, Proteins, Kits and Reagents for Life Science

311 587 ₸ Product size

500 µl 311 587 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Human Nuclear Antigen antibody [235-1] (ab191181)

Key features and details

Mouse monoclonal [235-1] to Human Nuclear Antigen
Suitable for: ICC, Flow Cyt, IHC-Fr
Reacts with: Human, Non human primates
Isotype: IgG1

Product name

Anti-Human Nuclear Antigen antibody [235-1]
See all Human Nuclear Antigen primary antibodies
Description

Mouse monoclonal [235-1] to Human Nuclear Antigen
Host species

Mouse
Specificity

ab191181 recognizes an antigen associated with the nuclei in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in frozen tissue sections. It can be used with paraformaldehyde-fixed frozen tissue or cell preparations.

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC	Human
WB	Human

See all applications and species data

Immunogen

Tissue, cells or virus corresponding to Human Nuclear Antigen. (Immunogen: Nuclei of Human myeloid leukemia biopsy cells).
Positive control
- IHC-Fr: Human tonsil tissue; ICC: MCF and K562 cells; Flow Cyt: Raji, MCF7, K562, HeLa, and Jurkat cells.
General notes

ab190710 is recommended for IHC-P with paraffin embedded sections.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

235-1
Isotype

IgG1
Light chain type

kappa
Research areas

Immunocytochemistry - Anti-Human Nuclear Antigen antibody [235-1] (ab191181)

PFA-fixed MCF-7 (human breast adenocarcinoma cell line) cells stained for Human Nuclear Antigen using ab191181 at 2 ug/ml in ICC.
Flow Cytometry - Anti-Human Nuclear Antigen antibody [235-1] (ab191181)

Flow Cytometric analysis of paraformaldehyde-fixed Raji (human Burkitt's lymphoma cell line) cells staining Human Nuclear Antigen using ab191181 at 2 ug/million cells, followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).
Immunohistochemistry (Frozen sections) - Anti-Human Nuclear Antigen antibody [235-1] (ab191181) Rockne et al PLoS One. 2018 Aug 2;13(8):e0199967. doi: 10.1371/journal.pone.0199967. eCollection 2018. Fig 1. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

Characterization of LM-NSC008 cells.

Visualization of LM-NSC008 cells in naïve non-tumor bearing mouse brain 6 and 9 months post administration. (A) Immunohistochemistry (IHC) staining of LM-NSC008 cells (stably expressing eGFP/Ffluc) with anti-HNA antibodies (NSC injection site, white arrows indicate the LM-NSC008 cells, scale bar 100 μm). (1a-1d) Insets show LM-NSC008 cells co-expressing HNA and eGFP proteins (inset scale bars [1a-1f] 2 μm).
Immunohistochemistry (Frozen sections) - Anti-Human Nuclear Antigen antibody [235-1] (ab191181)

Cold acetone-fixed frozen human tonsil tissue stained for Human Nuclear Antigen with ab191181 at 0.5 µg/ml (30 mins, RT) in immunohistochemical analysis. Visualised using a HRP-Polymer detection system.
Immunocytochemistry - Anti-Human Nuclear Antigen antibody [235-1] (ab191181)

PFA-fixed K562 (human chronic myelogenous leukemia cell line from bone marrow ) cells stained for Human Nuclear Antigen using ab191181 at 2 ug/ml in ICC.
Flow Cytometry - Anti-Human Nuclear Antigen antibody [235-1] (ab191181)

Flow Cytometric analysis of paraformaldehyde-fixed MCF-7 (human breast adenocarcinoma cell line) cells staining Human Nuclear Antigen using ab191181 at 2 ug/million cells, followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).
Flow Cytometry - Anti-Human Nuclear Antigen antibody [235-1] (ab191181)

Flow Cytometric analysis of paraformaldehyde-fixed K562 (human chronic myelogenous leukemia cell line from bone marrow ) cells staining Human Nuclear Antigen using ab191181 at 2 ug/million cells, followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).
Flow Cytometry - Anti-Human Nuclear Antigen antibody [235-1] (ab191181)

Flow Cytometric analysis of paraformaldehyde-fixed Jurkat (human T cell leukemia cell line from peripheral blood) cells staining Human Nuclear Antigen using ab191181 at 2 ug/million cells, followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).
Flow Cytometry - Anti-Human Nuclear Antigen antibody [235-1] (ab191181)

Flow Cytometric analysis of paraformaldehyde-fixed HeLa (human lymphoblastic leukemia cell line) cells staining Human Nuclear Antigen using ab191181 at 2 ug/million cells, followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com