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Anti-Human Nuclear Antigen antibody [235-1] (ab191181)

Key features and details

  • Mouse monoclonal [235-1] to Human Nuclear Antigen
  • Suitable for: ICC, Flow Cyt, IHC-Fr
  • Reacts with: Human, Non human primates
  • Isotype: IgG1

Overview

  • Product name

    Anti-Human Nuclear Antigen antibody [235-1]
    See all Human Nuclear Antigen primary antibodies

  • Description

    Mouse monoclonal [235-1] to Human Nuclear Antigen

  • Host species

    Mouse

  • Specificity

    ab191181 recognizes an antigen associated with the nuclei in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in frozen tissue sections. It can be used with paraformaldehyde-fixed frozen tissue or cell preparations.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Tissue, cells or virus corresponding to Human Nuclear Antigen. (Immunogen: Nuclei of Human myeloid leukemia biopsy cells).

  • Positive control

    • IHC-Fr: Human tonsil tissue; ICC: MCF and K562 cells; Flow Cyt: Raji, MCF7, K562, HeLa, and Jurkat cells.

  • General notes

    ab190710 is recommended for IHC-P with paraffin embedded sections.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.05% Sodium azide
    Constituents: PBS, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    235-1

  • Isotype

    IgG1

  • Light chain type

    kappa

  • Research areas

Images

  • Immunocytochemistry - Anti-Human Nuclear Antigen antibody [235-1] (ab191181)
    Immunocytochemistry - Anti-Human Nuclear Antigen antibody [235-1] (ab191181)
    PFA-fixed MCF-7 (human breast adenocarcinoma cell line) cells stained for Human Nuclear Antigen using ab191181 at 2 ug/ml in ICC.
  • Flow Cytometry - Anti-Human Nuclear Antigen antibody [235-1] (ab191181)
    Flow Cytometry - Anti-Human Nuclear Antigen antibody [235-1] (ab191181)
    Flow Cytometric analysis of paraformaldehyde-fixed Raji (human Burkitt's lymphoma cell line) cells staining Human Nuclear Antigen using ab191181 at 2 ug/million cells, followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).
  • Immunohistochemistry (Frozen sections) - Anti-Human Nuclear Antigen antibody [235-1] (ab191181)
    Immunohistochemistry (Frozen sections) - Anti-Human Nuclear Antigen antibody [235-1] (ab191181) Rockne et al PLoS One. 2018 Aug 2;13(8):e0199967. doi: 10.1371/journal.pone.0199967. eCollection 2018. Fig 1. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Characterization of LM-NSC008 cells.

    Visualization of LM-NSC008 cells in naïve non-tumor bearing mouse brain 6 and 9 months post administration. (A) Immunohistochemistry (IHC) staining of LM-NSC008 cells (stably expressing eGFP/Ffluc) with anti-HNA antibodies (NSC injection site, white arrows indicate the LM-NSC008 cells, scale bar 100 μm). (1a-1d) Insets show LM-NSC008 cells co-expressing HNA and eGFP proteins (inset scale bars [1a-1f] 2 μm).

     

  • Immunohistochemistry (Frozen sections) - Anti-Human Nuclear Antigen antibody [235-1] (ab191181)
    Immunohistochemistry (Frozen sections) - Anti-Human Nuclear Antigen antibody [235-1] (ab191181)

    Cold acetone-fixed frozen human tonsil tissue stained for Human Nuclear Antigen with ab191181 at 0.5 µg/ml (30 mins, RT) in immunohistochemical analysis. Visualised using a HRP-Polymer detection system.

  • Immunocytochemistry - Anti-Human Nuclear Antigen antibody [235-1] (ab191181)
    Immunocytochemistry - Anti-Human Nuclear Antigen antibody [235-1] (ab191181)
    PFA-fixed K562 (human chronic myelogenous leukemia cell line from bone marrow ) cells stained for Human Nuclear Antigen using ab191181 at 2 ug/ml in ICC.
  • Flow Cytometry - Anti-Human Nuclear Antigen antibody [235-1] (ab191181)
    Flow Cytometry - Anti-Human Nuclear Antigen antibody [235-1] (ab191181)
    Flow Cytometric analysis of paraformaldehyde-fixed MCF-7 (human breast adenocarcinoma cell line) cells staining Human Nuclear Antigen using ab191181 at 2 ug/million cells, followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).
  • Flow Cytometry - Anti-Human Nuclear Antigen antibody [235-1] (ab191181)
    Flow Cytometry - Anti-Human Nuclear Antigen antibody [235-1] (ab191181)
    Flow Cytometric analysis of paraformaldehyde-fixed K562 (human chronic myelogenous leukemia cell line from bone marrow ) cells staining Human Nuclear Antigen using ab191181 at 2 ug/million cells, followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).
  • Flow Cytometry - Anti-Human Nuclear Antigen antibody [235-1] (ab191181)
    Flow Cytometry - Anti-Human Nuclear Antigen antibody [235-1] (ab191181)
    Flow Cytometric analysis of paraformaldehyde-fixed Jurkat (human T cell leukemia cell line from peripheral blood) cells staining Human Nuclear Antigen using ab191181 at 2 ug/million cells, followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).
  • Flow Cytometry - Anti-Human Nuclear Antigen antibody [235-1] (ab191181)
    Flow Cytometry - Anti-Human Nuclear Antigen antibody [235-1] (ab191181)
    Flow Cytometric analysis of paraformaldehyde-fixed HeLa (human lymphoblastic leukemia cell line) cells staining Human Nuclear Antigen using ab191181 at 2 ug/million cells, followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).

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