For immunofluorescence analysis, THP1 cells were fixed and permeabilized for detection of endogenous CCR5 using Anti- CCR5 Recombinant Rabbit Multiclonal Antibody (ab277802, 2 μ/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal^™ Secondary Antibody, Alexa Fluor^® 488 conjugate (1/2000). Panel a) shows representative cells that were stained for detection and localization of CCR5 protein (green), Panel b) is stained for nuclei (blue) using SlowFade^® Gold Antifade Mountant with DAPI. Panel c) represents cytoskeletal F-actin staining using Alexa Fluor^® 555 Rhodamine Phalloidin (1/300). Panel d) is a composite image of Panels a, b and c clearly demonstrating membrane localization of CCR5. Panel e) represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.