Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PWP1 antibody [EPR16131] - C-terminal (ab190795)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16131] to PWP1 - C-terminal
  • Suitable for: WB, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PWP1 antibody [EPR16131] - C-terminal
    See all PWP1 primary antibodies

  • Description

    Rabbit monoclonal [EPR16131] to PWP1 - C-terminal

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • 293, Jurkat, HepG2, HeLa, NIH-3T3 cell lysates; Jurkat cells

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR16131

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-PWP1 antibody [EPR16131] - C-terminal (ab190795)
    Western blot - Anti-PWP1 antibody [EPR16131] - C-terminal (ab190795)
    All lanes : Anti-PWP1 antibody [EPR16131] - C-terminal (ab190795) at 1/10000 dilution

    Lane 1 : 293 cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : Hela cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 56 kDa
    Observed band size: 79 kDa
    why is the actual band size different from the predicted?

  • Western blot - Anti-PWP1 antibody [EPR16131] - C-terminal (ab190795)
    Western blot - Anti-PWP1 antibody [EPR16131] - C-terminal (ab190795)
    All lanes : Anti-PWP1 antibody [EPR16131] - C-terminal (ab190795) at 1/1000 dilution

    Lane 1 : C6 cell lysate
    Lane 2 : NIH-3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 56 kDa
    Observed band size: 79 kDa why is the actual band size different from the predicted?

  • Flow Cytometry - Anti-PWP1 antibody [EPR16131] - C-terminal (ab190795)
    Flow Cytometry - Anti-PWP1 antibody [EPR16131] - C-terminal (ab190795)

    Flow cytometry analysis of PWP1 expression in Jurkat cells using ab190795 at 1/180 dilution (red) and a rabbit IgG as negative control (green). A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody.

  • Anti-PWP1 antibody [EPR16131] - C-terminal (ab190795)
    Anti-PWP1 antibody [EPR16131] - C-terminal (ab190795)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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