Anti-PWP1 antibody [EPR16131] - C-terminal (ab190795)
![Anti-PWP1 antibody [EPR16131] - C-terminal (ab190795)](https://www.abcam.com/ps/products/190/ab190795/Images/ab190795-226982-wb.jpg "Anti-PWP1 antibody [EPR16131] - C-terminal (ab190795)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16131] to PWP1 - C-terminal
- Suitable for: WB, Flow Cyt
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-PWP1 antibody [EPR16131] - C-terminal
See all PWP1 primary antibodies -
Description
Rabbit monoclonal [EPR16131] to PWP1 - C-terminal -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanWB RatHuman
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- 293, Jurkat, HepG2, HeLa, NIH-3T3 cell lysates; Jurkat cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16131 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PWP1 antibody [EPR16131] - C-terminal (ab190795)All lanes : Anti-PWP1 antibody [EPR16131] - C-terminal (ab190795) at 1/10000 dilution
Lane 1 : 293 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : Hela cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 56 kDa
Observed band size: 79 kDa why is the actual band size different from the predicted?
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![Western blot - Anti-PWP1 antibody [EPR16131] - C-terminal (ab190795)](https://www.abcam.com/ps/products/190/ab190795/Images/ab190795-226981-wb.jpg)
Western blot - Anti-PWP1 antibody [EPR16131] - C-terminal (ab190795)All lanes : Anti-PWP1 antibody [EPR16131] - C-terminal (ab190795) at 1/1000 dilution
Lane 1 : C6 cell lysate
Lane 2 : NIH-3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 56 kDa
Observed band size: 79 kDa why is the actual band size different from the predicted?
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![Flow Cytometry - Anti-PWP1 antibody [EPR16131] - C-terminal (ab190795)](https://www.abcam.com/ps/products/190/ab190795/Images/ab190795-226980-flow.jpg)
Flow Cytometry - Anti-PWP1 antibody [EPR16131] - C-terminal (ab190795)Flow cytometry analysis of PWP1 expression in Jurkat cells using ab190795 at 1/180 dilution (red) and a rabbit IgG as negative control (green). A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody.
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![Anti-PWP1 antibody [EPR16131] - C-terminal (ab190795)](https://www.abcam.com/ps/products/190/ab190795/Images/ab190795-4-benefits-of-recombinant-antibodies.png)
Anti-PWP1 antibody [EPR16131] - C-terminal (ab190795)
