Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-RIP antibody [EPR4689-100] - BSA and Azide free (ab227843)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4689-100] to RIP - BSA and Azide free
  • Suitable for: WB, Flow Cyt
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-RIP antibody [EPR4689-100] - BSA and Azide free
    See all RIP primary antibodies

  • Description

    Rabbit monoclonal [EPR4689-100] to RIP - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HeLa cells and cell lysates; Raji cell lysates.

  • General notes

    ab227843 is the carrier-free version of ab178420 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab227843 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Images

  • Flow Cytometry - Anti-RIP antibody [EPR4689-100] - BSA and Azide free (ab227843)
    Flow Cytometry - Anti-RIP antibody [EPR4689-100] - BSA and Azide free (ab227843)

    This Flow Cyt data was generated using the same anti-RIP antibody clone, EPR4689-100, in a different buffer formulation (cat# ab178420).

    Flow Cytometric analysis of permeabilized HeLa cells labeling RIP with ab178420 at 1/10 dilution (red) compared with a rabbit IgG negative control (green).

  • Western blot - Anti-RIP antibody [EPR4689-100] - BSA and Azide free (ab227843)
    Western blot - Anti-RIP antibody [EPR4689-100] - BSA and Azide free (ab227843)

    This WB data was generated using the same anti-RIP antibody clone, EPR4689-100, in a different buffer formulation (cat# ab178420).

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: RIP knockout HAP1 cell lysate (20 µg) 
    Lane 3: HeLa cell lysate (20 µg)
    Lane 4: Raji cell lysate (20 µg) 
    Lanes 1 to 4: Merged signal (red and green). Green - ab178420 observed at 78 kDa. Red - loading control, ab8245, observed at 37 kDa.
    ab178420 was shown to specifically react with RIP when RIP knockout samples were used. Wild-type and RIP knockout samples were subjected to SDS-PAGE. ab178420 and ab8245 (loading control to GAPDH) were both diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) ab216776 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

  • Anti-RIP antibody [EPR4689-100] - BSA and Azide free (ab227843)
    Anti-RIP antibody [EPR4689-100] - BSA and Azide free (ab227843)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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