Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Estrogen Inducible Protein pS2 antibody [EPR3972] - BSA and Azide free (ab239908)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3972] to Estrogen Inducible Protein pS2 - BSA and Azide free
  • Suitable for: IP, ICC/IF, Flow Cyt, IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-Estrogen Inducible Protein pS2 antibody [EPR3972] - BSA and Azide free
    See all Estrogen Inducible Protein pS2 primary antibodies

  • Description

    Rabbit monoclonal [EPR3972] to Estrogen Inducible Protein pS2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, ICC/IF, Flow Cyt, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab239908 is the carrier-free version of ab92377 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab239908 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Inducible Protein pS2 antibody [EPR3972] - BSA and Azide free (ab239908)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Inducible Protein pS2 antibody [EPR3972] - BSA and Azide free (ab239908)

    ab92377 staining Estrogen Inducible Protein pS2 in Formalin-fixed, Paraffin-embedded Human breast carcinoma tissue (A) and Human stomach tissue (B) at 1/100 dilution. Detection: DAB staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92377).

  • Flow Cytometry - Anti-Estrogen Inducible Protein pS2 antibody [EPR3972] - BSA and Azide free (ab239908)
    Flow Cytometry - Anti-Estrogen Inducible Protein pS2 antibody [EPR3972] - BSA and Azide free (ab239908)

    Flow Cytometry analysis of MCF-7 (human breast carcinoma) cells labeling Estrogen Inducible Protein pS2 with unpurified ab92377 at 1/20 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92377).

  • Immunocytochemistry/ Immunofluorescence - Anti-Estrogen Inducible Protein pS2 antibody [EPR3972] - BSA and Azide free (ab239908)
    Immunocytochemistry/ Immunofluorescence - Anti-Estrogen Inducible Protein pS2 antibody [EPR3972] - BSA and Azide free (ab239908)

    ICC/IF image of ab92377 stained MCF7 cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab92377, 1/100 dilution) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92377).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Inducible Protein pS2 antibody [EPR3972] - BSA and Azide free (ab239908)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Inducible Protein pS2 antibody [EPR3972] - BSA and Azide free (ab239908)

    ab92377 showing positive staining in Ovarian carcinoma tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92377).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Inducible Protein pS2 antibody [EPR3972] - BSA and Azide free (ab239908)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Inducible Protein pS2 antibody [EPR3972] - BSA and Azide free (ab239908)

    ab92377 showing negative staining in Normal breast tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92377).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Inducible Protein pS2 antibody [EPR3972] - BSA and Azide free (ab239908)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Inducible Protein pS2 antibody [EPR3972] - BSA and Azide free (ab239908)

    ab92377 showing negative staining in Normal kidney tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92377).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Inducible Protein pS2 antibody [EPR3972] - BSA and Azide free (ab239908)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Inducible Protein pS2 antibody [EPR3972] - BSA and Azide free (ab239908)

    ab92377 showing positive staining in Ductal infiltrating breast carcinoma tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92377).

  • OI-RD Scanning - Anti-Estrogen Inducible Protein pS2 antibody [EPR3972] - BSA and Azide free (ab239908)
    OI-RD Scanning - Anti-Estrogen Inducible Protein pS2 antibody [EPR3972] - BSA and Azide free (ab239908)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92377).

  • Anti-Estrogen Inducible Protein pS2 antibody [EPR3972] - BSA and Azide free (ab239908)
    Anti-Estrogen Inducible Protein pS2 antibody [EPR3972] - BSA and Azide free (ab239908)

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