Anti-Carboxypeptidase A1+A2+B antibody [EPR12087(B)] - BSA and Azide free (ab250379)
![Anti-Carboxypeptidase A1+A2+B antibody [EPR12087(B)] - BSA and Azide free (ab250379)](https://www.abcam.com/ps/products/250/ab250379/Images/ab250379-407810-anti-carboxypeptidase-a1-a2-b-antibody-epr12087-b-bsa-and-azide-free-western-blot.jpg "Anti-Carboxypeptidase A1+A2+B antibody [EPR12087(B)] - BSA and Azide free (ab250379)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12087(B)] to Carboxypeptidase A1+A2+B - BSA and Azide free
- Suitable for: IHC-P, ICC, WB
- Reacts with: Mouse, Rat, Human
Overview
-
Product name
Anti-Carboxypeptidase A1+A2+B antibody [EPR12087(B)] - BSA and Azide free
See all Carboxypeptidase A1+A2+B primary antibodies -
Description
Rabbit monoclonal [EPR12087(B)] to Carboxypeptidase A1+A2+B - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
General notes
Ab250379 is the carrier-free version of ab181146. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab250379 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12087(B) -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-Carboxypeptidase A1+A2+B antibody [EPR12087(B)] - BSA and Azide free (ab250379)Anti-Carboxypeptidase A1+A2+B antibody [EPR12087(B)] (ab181146) at 1/5000 dilution + Human pancreas tissue lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 47 kDaThis data was developed using ab181146, the same antibody clone in a different buffer formulation.
-
![Immunocytochemistry - Anti-Carboxypeptidase A1+A2+B antibody [EPR12087(B)] - BSA and Azide free (ab250379)](https://www.abcam.com/ps/products/250/ab250379/Images/ab250379-3-anti-carboxypeptidase-a1-a2-b-antibody-epr12087-b-bsa-and-azide-free-immunocytochemistry.jpg)
Immunocytochemistry - Anti-Carboxypeptidase A1+A2+B antibody [EPR12087(B)] - BSA and Azide free (ab250379)This data was developed using ab181146, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of BxPC3 cells (paraformaldehyde-fixed, 4%) labeling Carboxypeptidase A with ab181146 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary at 1/200 dilution and counter-stained with DAPI (blue). -
![Western blot - Anti-Carboxypeptidase A1+A2+B antibody [EPR12087(B)] - BSA and Azide free (ab250379)](https://www.abcam.com/ps/products/250/ab250379/Images/ab250379-402676-anti-carboxypeptidase-a1-a2-b-antibody-epr12087-b-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-Carboxypeptidase A1+A2+B antibody [EPR12087(B)] - BSA and Azide free (ab250379)All lanes : Anti-Carboxypeptidase A1+A2+B antibody [EPR12087(B)] (ab181146) at 1/1000 dilution
Lane 1 : Rat pancreas lysate
Lane 2 : Mouse pancreas lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 47 kDaThis data was developed using ab181146, the same antibody clone in a different buffer formulation.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A1+A2+B antibody [EPR12087(B)] - BSA and Azide free (ab250379)](https://www.abcam.com/ps/products/250/ab250379/Images/ab250379-1-anti-carboxypeptidase-a1-a2-b-antibody-epr12087-b-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A1+A2+B antibody [EPR12087(B)] - BSA and Azide free (ab250379)This data was developed using ab181146, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling Carboxypeptidase A with ab181146 at 1/500 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol. -
![Anti-Carboxypeptidase A1+A2+B antibody [EPR12087(B)] - BSA and Azide free (ab250379)](https://www.abcam.com/ps/products/250/ab250379/Images/ab250379-5-benefits-of-recombinant-antibodies.png)
Anti-Carboxypeptidase A1+A2+B antibody [EPR12087(B)] - BSA and Azide free (ab250379)