Anti-Cyclin K antibody (ab85854)
Key features and details
- Rabbit polyclonal to Cyclin K
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Cyclin K antibody
See all Cyclin K primary antibodies -
Description
Rabbit polyclonal to Cyclin K -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human Cyclin K aa 530-580.
Database link: NP_001092872.1 -
Positive control
- HeLa and 293T whole cell lysates.
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General notes
Previously labelled as CCNK.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab85854 was affinity purified using an epitope specific to Cyclin K immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Cyclin K antibody (ab85854)All lanes : Anti-Cyclin K antibody (ab85854) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 66 kDa
Exposure time: 3 minutes
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Immunoprecipitation - Anti-Cyclin K antibody (ab85854)Detection of human Cyclin K by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Cyclin K antibody ab85854 used for IP at 6 µg per reaction. Cyclin K was also immunoprecipitated by a previous lot of this antibody. For blotting immunoprecipitated Cyclin K, ab85854 was used at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 10 seconds.
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Western blot - Anti-Cyclin K antibody (ab85854)All lanes : Anti-Cyclin K antibody (ab85854) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 66 kDa
Observed band size: 64 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes