Anti-Leptin antibody [AT7E10] (ab219260)
![Anti-Leptin antibody [AT7E10] (ab219260)](https://www.abcam.com/ps/products/219/ab219260/Images/ab219260-275687-anti-leptin-antibody-at7e10-western-blot.jpg "Anti-Leptin antibody [AT7E10] (ab219260)")
Key features and details
- Mouse monoclonal [AT7E10] to Leptin
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG2b
Overview
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Product name
Anti-Leptin antibody [AT7E10]
See all Leptin primary antibodies -
Description
Mouse monoclonal [AT7E10] to Leptin -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Sheep, Goat, Cow, Cat, Pig, Chimpanzee, Rhesus monkey, Gorilla
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Immunogen
Recombinant full length protein corresponding to Human Leptin aa 1 to the C-terminus. (Purified from E.coli).
Database link: P41159 -
Positive control
- Leptin transfected 293T cell lysate; HeLa cells.
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General notes
Clone AT7E10 is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Leptin protein.
This product was changed from ascites to tissue culture supernatant on 08/08/2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 89% PBS, 10% Glycerol -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
AT7E10 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Images
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Western blot - Anti-Leptin antibody [AT7E10] (ab219260)All lanes : Anti-Leptin antibody [AT7E10] (ab219260) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : Leptin transfected 293T cell lysate
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : Goat anti-Mouse secondary antibody conjugated to HRP
Developed using the ECL technique.
Predicted band size: 19 kDaThis image was generated using the ascites version of the product.
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![Immunocytochemistry/ Immunofluorescence - Anti-Leptin antibody [AT7E10] (ab219260)](https://www.abcam.com/ps/products/219/ab219260/Images/ab219260-275688-anti-leptin-antibody-at7e10-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Leptin antibody [AT7E10] (ab219260)Immunofluorescent analysis of HeLa cells labeling Leptin with ab219260 at 1/100 dilution, followed by goat anti-mouse IgG-Alexa fluor 488 conjugate (green). The lower panel shows cells counterstained with DAPI for nuclear staining (blue).
This image was generated using the ascites version of the product.
