Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Leptin antibody [AT7E10] (ab219260)

Key features and details

  • Mouse monoclonal [AT7E10] to Leptin
  • Suitable for: WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG2b

Overview

  • Product name

    Anti-Leptin antibody [AT7E10]
    See all Leptin primary antibodies

  • Description

    Mouse monoclonal [AT7E10] to Leptin

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Sheep, Goat, Cow, Cat, Pig, Chimpanzee, Rhesus monkey, Gorilla

  • Immunogen

    Recombinant full length protein corresponding to Human Leptin aa 1 to the C-terminus. (Purified from E.coli).
    Database link: P41159

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Leptin transfected 293T cell lysate; HeLa cells.

  • General notes

    Clone AT7E10 is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Leptin protein.

    This product was changed from ascites to tissue culture supernatant on 08/08/2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Properties

Images

  • Western blot - Anti-Leptin antibody [AT7E10] (ab219260)
    Western blot - Anti-Leptin antibody [AT7E10] (ab219260)
    All lanes : Anti-Leptin antibody [AT7E10] (ab219260) at 1/1000 dilution

    Lane 1 : 293T cell lysate
    Lane 2 : Leptin transfected 293T cell lysate

    Lysates/proteins at 40 µg per lane.

    Secondary
    All lanes : Goat anti-Mouse secondary antibody conjugated to HRP

    Developed using the ECL technique.

    Predicted band size: 19 kDa



    This image was generated using the ascites version of the product.

  • Immunocytochemistry/ Immunofluorescence - Anti-Leptin antibody [AT7E10] (ab219260)
    Immunocytochemistry/ Immunofluorescence - Anti-Leptin antibody [AT7E10] (ab219260)

    Immunofluorescent analysis of HeLa cells labeling Leptin with ab219260 at 1/100 dilution, followed by goat anti-mouse IgG-Alexa fluor 488 conjugate (green). The lower panel shows cells counterstained with DAPI for nuclear staining (blue).

    This image was generated using the ascites version of the product.

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