Anti-Leptin antibody [EPR16848-34] (ab181298)
![Anti-Leptin antibody [EPR16848-34] (ab181298)](https://www.abcam.com/ps/products/181/ab181298/Images/ab181298-331391-anti-leptin-antibody-epr16848-34-blocking.jpg "Anti-Leptin antibody [EPR16848-34] (ab181298)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16848-34] to Leptin
- Suitable for: Blocking
- Reacts with: Human
Overview
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Product name
Anti-Leptin antibody [EPR16848-34]
See all Leptin primary antibodies -
Description
Rabbit monoclonal [EPR16848-34] to Leptin -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species BL Human
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Immunogen
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Blocking Assay: HEK-293 cells co-transfected with a plasmid encoding the full-length mouse leptin receptor, pRL-CMV and pGL4.47[luc2P/SIE/Hygro] Vectors.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16848-34 -
Isotype
IgG -
Research areas
Images
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Blocking - Anti-Leptin antibody [EPR16848-34] (ab181298) The data was provided by our collaborator Dr. Bing Sun, Chinese Academy of Sciences.
HEK-293 cells were co-transfected with a plasmid encoding the full-length mouse leptin receptor, pRL-CMV and pGL4.47[luc2P/SIE/Hygro] Vectors. Dual-Luciferase® Reporter Assay was used to test the leptin signaling.
ab181298 (0, 50, 100 µg/ml) was incubated with recombinant mouse leptin in a total of 500ul culture medium at the final concentration of 100, 50, 25, and 12.5 ng/ml for 2h at 37℃ before adding to transfected cells.
Leptin without incubating with ab181298 as the positive control (green) shows the maximal signal of the leptin and leptin receptor interaction, whereas the base line signal (without adding leptin and ab181298 to the system) serves as the negative control (purple).
The data demonstrates that the interaction of leptin and leptin receptor can be blocked by ab181298 resulting in decreased florescence signals.
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![Blocking - Anti-Leptin antibody [EPR16848-34] (ab181298)](https://www.abcam.com/ps/products/181/ab181298/Images/ab181298-331392-anti-leptin-antibody-epr16848-34-blocking.jpg)
Blocking - Anti-Leptin antibody [EPR16848-34] (ab181298) The data was provided by our collaborator Dr. Bing Sun, Chinese Academy of Sciences.HEK-293 cells were co-transfected with a plasmid encoding full length human leptin receptor, pRL-CMV and pGL4.47[luc2P/SIE/Hygro] Vectors. Dual-Luciferase® Reporter Assay was used to test the leptin signaling.
ab181298 (0, 50, 100 µg/ml) was incubated with recombinant mouse leptin in a total of 500ul culture medium at the final concentration of 100, 50, 25, and 12.5 ng/ml for 2h at 37℃ before adding to transfected cells.
Leptin without incubating with ab181298 as the positive control (green) shows the maximal signal of the leptin and leptin receptor interaction, whereas the base line signal (without adding leptin and ab181298 to the system) serves as the negative control (purple).
The data demonstrates that the interaction of leptin and leptin receptor can be blocked by ab181298 resulting in decreased florescence signals.
-
![Anti-Leptin antibody [EPR16848-34] (ab181298)](https://www.abcam.com/ps/products/181/ab181298/Images/ab181298-3-benefits-of-recombinant-antibodies.png)
Anti-Leptin antibody [EPR16848-34] (ab181298)
