Anti-xCT antibody (ab216876)
Key features and details
- Rabbit polyclonal to xCT
- Suitable for: WB, ICC/IF, Flow Cyt
- Reacts with: Mouse, Human, Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-xCT antibody
See all xCT primary antibodies -
Description
Rabbit polyclonal to xCT -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Human, Recombinant fragment -
Immunogen
This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: A549, HCT 116 and HeLa whole cell lysates. ICC/IF: PC-3 cells. Flow Cytometry: Human breast carcinoma cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-xCT antibody (ab216876) at 1/1000 dilution
Lane 1 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 2 : HCT 116 (human colorectal carcinoma cell line) whole cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : anti-rabbit secondary DyLight 488 antibody at 1/20000 dilution
Predicted band size: 55 kDa
Observed band size: 56 kDa why is the actual band size different from the predicted?Dilution buffer: 0.01% sodium azide in PBS.
xCT processing caused by dimerization, glycosylation, and/or phosphorylation may cause other bands.
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Immunofluorescent analysis of PC-3 (human prostate adenocarcinoma cell line) cells labeling xCT with ab216876 at 10 µg/mL for 1 hour, followed by anti-Rabbit IgG DyLight™ 488 Conjugated Preadsorbed secondary antibody at 5 µg/mL for 1 hour at RT (green). Cells were fixed with 100% methanol.
The nuclear counter stain is DAPI (blue).
A: DAPI
B: Primary followed by Anti-rabbit IgG Dylight™ 488
C: Merged images
D: Secondary only control
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Flow cytometric analysis of human breast carcinoma cells labeling xCT with ab216876 at 1 µg/mL for 1 hour at 4°C. Rabbit IgG Antibody Dylight™488 Conjugated pre-adsorbed secondary antibody was used at 1 µg/mL for one hour on ice.
Left panels: Unstained.
Middle panels: Secondary antibody only.
Right panels: Primary and secondary antibodies.
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All lanes : Anti-xCT antibody (ab216876) at 1/1000 dilution
Lane 1 : Recombinant Bcl3 (unrelated negative control)
Lane 2 : NIH/3T3 (mouse embryonic fibroblast cell line) whole cell lysate
Lane 3 : A549 (human lung carcinoma cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : anti-rabbit secondary HRP antibody at 1/70000 dilution
Predicted band size: 55 kDa
Observed band size: 56 kDa why is the actual band size different from the predicted?Exposure time: 15-30 seconds.
Blocking buffer: 5% BSA.
Dilution buffer: 0.01% sodium azide in PBS.
xCT processing caused by dimerization, glycosylation, and/or phosphorylation may cause other bands.