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Signal Transduction Metabolism Amino Acids

Anti-xCT antibody (ab216876)

Price and availability

278 083 ₸

Availability

Order now and get it on Thursday February 25, 2021

Anti-xCT antibody (ab216876)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to xCT
  • Suitable for: WB, ICC/IF, Flow Cyt
  • Reacts with: Mouse, Human, Recombinant fragment
  • Isotype: IgG

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Overview

  • Product name

    Anti-xCT antibody
    See all xCT primary antibodies
  • Description

    Rabbit polyclonal to xCT
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Human, Recombinant fragment
  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: A549, HCT 116 and HeLa whole cell lysates. ICC/IF: PC-3 cells. Flow Cytometry: Human breast carcinoma cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Amino Acids
    • Signal Transduction
    • Metabolism
    • Plasma Membrane
    • Channels
    • Cell Biology
    • Other Antibodies
    • Oxidative Stress
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Amino acid metabolism
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Oxidative stress

Images

  • Western blot - Anti-xCT antibody (ab216876)
    Western blot - Anti-xCT antibody (ab216876)
    All lanes : Anti-xCT antibody (ab216876) at 1/1000 dilution

    Lane 1 : A549 (human lung carcinoma cell line) whole cell lysate
    Lane 2 : HCT 116 (human colorectal carcinoma cell line) whole cell lysate
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : anti-rabbit secondary DyLight 488 antibody at 1/20000 dilution

    Predicted band size: 55 kDa
    Observed band size: 56 kDa
    why is the actual band size different from the predicted?



    Dilution buffer: 0.01% sodium azide in PBS.

    xCT processing caused by dimerization, glycosylation, and/or phosphorylation may cause other bands.

  • Immunocytochemistry/ Immunofluorescence - Anti-xCT antibody (ab216876)
    Immunocytochemistry/ Immunofluorescence - Anti-xCT antibody (ab216876)

    Immunofluorescent analysis of PC-3 (human prostate adenocarcinoma cell line) cells labeling xCT with ab216876  at 10 µg/mL for 1 hour, followed by anti-Rabbit IgG DyLight™ 488 Conjugated Preadsorbed secondary antibody at 5 µg/mL for 1 hour at RT (green). Cells were fixed with 100% methanol.

    The nuclear counter stain is DAPI (blue).

    A: DAPI

    B: Primary followed by Anti-rabbit IgG Dylight™ 488

    C: Merged images

    D: Secondary only control

  • Flow Cytometry - Anti-xCT antibody (ab216876)
    Flow Cytometry - Anti-xCT antibody (ab216876)

    Flow cytometric analysis of human breast carcinoma cells labeling xCT with ab216876 at 1 µg/mL for 1 hour at 4°C. Rabbit IgG Antibody Dylight™488 Conjugated pre-adsorbed secondary antibody was used at 1 µg/mL for one hour on ice.

    Left panels: Unstained.

    Middle panels: Secondary antibody only.

    Right panels: Primary and secondary antibodies.

  • Western blot - Anti-xCT antibody (ab216876)
    Western blot - Anti-xCT antibody (ab216876)
    All lanes : Anti-xCT antibody (ab216876) at 1/1000 dilution

    Lane 1 : Recombinant Bcl3 (unrelated negative control)
    Lane 2 : NIH/3T3 (mouse embryonic fibroblast cell line) whole cell lysate
    Lane 3 : A549 (human lung carcinoma cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : anti-rabbit secondary HRP antibody at 1/70000 dilution

    Predicted band size: 55 kDa
    Observed band size: 56 kDa why is the actual band size different from the predicted?



    Exposure time: 15-30 seconds.

    Blocking buffer: 5% BSA.

    Dilution buffer: 0.01% sodium azide in PBS.

    xCT processing caused by dimerization, glycosylation, and/or phosphorylation may cause other bands.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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