Anti-xCT antibody [EPR8290(2)] - BSA and Azide free (ab238969)
![Anti-xCT antibody [EPR8290(2)] - BSA and Azide free (ab238969)](https://www.abcam.com/ps/products/238/ab238969/Images/ab238969-3-ab175186IP1.jpg "Anti-xCT antibody [EPR8290(2)] - BSA and Azide free (ab238969)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8290(2)] to xCT - BSA and Azide free
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-xCT antibody [EPR8290(2)] - BSA and Azide free
See all xCT primary antibodies -
Description
Rabbit monoclonal [EPR8290(2)] to xCT - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: A549, HepG2, U-87 MG and BxPC-3 cell lysate. IP: HepG2 cells lysate.
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General notes
Ab238969 is the carrier-free version of ab175186. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab238969 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR8290(2) -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-xCT antibody [EPR8290(2)] - BSA and Azide free (ab238969)
ab175186 (purified) at 1:20 dilution (0.5µg) immunoprecipitating xCT in HepG2 whole cell lysate.
Lane 1 (input): HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate 10µg
Lane 2 (+): ab175186 & HepG2 whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab175186 in HepG2 whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, tissue culture supernatant and sodium azide (ab175186).
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![Immunoprecipitation - Anti-xCT antibody [EPR8290(2)] - BSA and Azide free (ab238969)](https://www.abcam.com/ps/products/238/ab238969/Images/ab238969-322820-anti-xct-antibody-epr82902-immunoprecipitation.jpg)
Immunoprecipitation - Anti-xCT antibody [EPR8290(2)] - BSA and Azide free (ab238969)Western blot analysis on immunoprecipitation pellet from (Lane 1) HepG2 cells lysate or (Lane 2) 1 X PBS (negative control) using ab175186 at a 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab175186).
This image was generated using the unpurified version of the product.
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![Western blot - Anti-xCT antibody [EPR8290(2)] - BSA and Azide free (ab238969)](https://www.abcam.com/ps/products/238/ab238969/Images/ab238969-317663-anti-xct-antibody-epr82902-western-blot.jpg)
Western blot - Anti-xCT antibody [EPR8290(2)] - BSA and Azide free (ab238969)All lanes : Anti-xCT antibody [EPR8290(2)] (ab175186) at 1/1000 dilution
Lane 1 : A549 (Human lung carcinoma cell line) cell lysates
Lane 2 : HepG2 (Human liver hepatocellular carcinoma cell line) cell lysates
Lane 3 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cell lysates
Lane 4 : BxPC-3 (Human pancreas adenocarcinoma cell line) cell lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 55 kDaThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, tissue culture supernatant and sodium azide (ab175186).
This image was generated using the unpurified version of the product.
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![Anti-xCT antibody [EPR8290(2)] - BSA and Azide free (ab238969)](https://www.abcam.com/ps/products/238/ab238969/Images/ab238969-4-benefits-of-recombinant-antibodies.png)
Anti-xCT antibody [EPR8290(2)] - BSA and Azide free (ab238969)
