Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-xCT antibody [EPR8290(2)] - BSA and Azide free (ab238969)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8290(2)] to xCT - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-xCT antibody [EPR8290(2)] - BSA and Azide free
    See all xCT primary antibodies

  • Description

    Rabbit monoclonal [EPR8290(2)] to xCT - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: A549, HepG2, U-87 MG and BxPC-3 cell lysate. IP: HepG2 cells lysate.

  • General notes

    Ab238969 is the carrier-free version of ab175186. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab238969 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunoprecipitation - Anti-xCT antibody [EPR8290(2)] - BSA and Azide free (ab238969)
    Immunoprecipitation - Anti-xCT antibody [EPR8290(2)] - BSA and Azide free (ab238969)

    ab175186 (purified) at 1:20 dilution (0.5µg) immunoprecipitating xCT in HepG2 whole cell lysate.
    Lane 1 (input): HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate 10µg
    Lane 2 (+): ab175186 & HepG2 whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab175186 in HepG2 whole cell lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, tissue culture supernatant and sodium azide (ab175186).

  • Immunoprecipitation - Anti-xCT antibody [EPR8290(2)] - BSA and Azide free (ab238969)
    Immunoprecipitation - Anti-xCT antibody [EPR8290(2)] - BSA and Azide free (ab238969)

    Western blot analysis on immunoprecipitation pellet from (Lane 1) HepG2 cells lysate or (Lane 2) 1 X PBS (negative control) using ab175186 at a 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab175186).

    This image was generated using the unpurified version of the product. 

  • Western blot - Anti-xCT antibody [EPR8290(2)] - BSA and Azide free (ab238969)
    Western blot - Anti-xCT antibody [EPR8290(2)] - BSA and Azide free (ab238969)
    All lanes : Anti-xCT antibody [EPR8290(2)] (ab175186) at 1/1000 dilution

    Lane 1 : A549 (Human lung carcinoma cell line) cell lysates
    Lane 2 : HepG2 (Human liver hepatocellular carcinoma cell line) cell lysates
    Lane 3 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cell lysates
    Lane 4 : BxPC-3 (Human pancreas adenocarcinoma cell line) cell lysates

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 55 kDa



    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, tissue culture supernatant and sodium azide (ab175186).

    This image was generated using the unpurified version of the product. 

  • Anti-xCT antibody [EPR8290(2)] - BSA and Azide free (ab238969)
    Anti-xCT antibody [EPR8290(2)] - BSA and Azide free (ab238969)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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