Anti-xCT antibody [EPR8290(2)] (ab175186)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8290(2)] to xCT
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-xCT antibody [EPR8290(2)]
See all xCT primary antibodies -
Description
Rabbit monoclonal [EPR8290(2)] to xCT -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human -
Immunogen
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Positive control
- WB: A549, HepG2, U87-MG and BxPC-3 cell lysates. Human, at and mouse heart tissue lysates IP: HepG2 cells lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR8290(2) -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-xCT antibody [EPR8290(2)] (ab175186) at 1/10000 dilution (Purified)
Lane 1 : A549 (Human lung carcinoma epithelial cell) whole cell lysates
Lane 2 : Human heart lysates
Lane 3 : Mouse heart lysates
Lane 4 : Rat heart lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 55 kDa
Observed band size: 55 kDa
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ab175186 (purified) at 1:20 dilution (0.5µg) immunoprecipitating xCT in HepG2 whole cell lysate.
Lane 1 (input): HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate 10µg
Lane 2 (+): ab175186 & HepG2 whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab175186 in HepG2 whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST. -
All lanes : Anti-xCT antibody [EPR8290(2)] (ab175186) at 1/1000 dilution
Lane 1 : A549 (Human lung carcinoma cell line) cell lysates
Lane 2 : HepG2 (Human liver hepatocellular carcinoma cell line) cell lysates
Lane 3 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cell lysates
Lane 4 : BxPC-3 (Human pancreas adenocarcinoma cell line) cell lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 55 kDaThis image was generated using the unpurified version of the product.
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Western blot analysis on immunoprecipitation pellet from (Lane 1) HepG2 cells lysate or (Lane 2) 1 X PBS (negative control) using ab175186 at a 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
This image was generated using the unpurified version of the product.
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