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Signal Transduction Metabolism Amino Acids

Anti-xCT antibody [EPR8290(2)] (ab175186)

Price and availability

328 339 ₸

Availability

Order now and get it on Tuesday March 02, 2021

Anti-xCT antibody [EPR8290(2)] (ab175186)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8290(2)] to xCT
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-xCT antibody [EPR8290(2)]
    See all xCT primary antibodies
  • Description

    Rabbit monoclonal [EPR8290(2)] to xCT
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human xCT aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q9UPY5
    (Peptide available as ab178606)

  • Positive control

    • WB: A549, HepG2, U87-MG and BxPC-3 cell lysates. Human, at and mouse heart tissue lysates IP: HepG2 cells lysate.
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR8290(2)
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Amino Acids
    • Signal Transduction
    • Metabolism
    • Plasma Membrane
    • Channels
    • Cell Biology
    • Other Antibodies
    • Oxidative Stress
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Amino acid metabolism
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Oxidative stress

Images

  • Western blot - Anti-xCT antibody [EPR8290(2)] (ab175186)
    Western blot - Anti-xCT antibody [EPR8290(2)] (ab175186)
    All lanes : Anti-xCT antibody [EPR8290(2)] (ab175186) at 1/10000 dilution (Purified)

    Lane 1 : A549 (Human lung carcinoma epithelial cell) whole cell lysates
    Lane 2 : Human heart lysates
    Lane 3 : Mouse heart lysates
    Lane 4 : Rat heart lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 55 kDa
    Observed band size: 55 kDa

  • Immunoprecipitation - Anti-xCT antibody [EPR8290(2)] (ab175186)
    Immunoprecipitation - Anti-xCT antibody [EPR8290(2)] (ab175186)

    ab175186 (purified) at 1:20 dilution (0.5µg) immunoprecipitating xCT in HepG2 whole cell lysate.
    Lane 1 (input): HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate 10µg
    Lane 2 (+): ab175186 & HepG2 whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab175186 in HepG2 whole cell lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

  • Western blot - Anti-xCT antibody [EPR8290(2)] (ab175186)
    Western blot - Anti-xCT antibody [EPR8290(2)] (ab175186)
    All lanes : Anti-xCT antibody [EPR8290(2)] (ab175186) at 1/1000 dilution

    Lane 1 : A549 (Human lung carcinoma cell line) cell lysates
    Lane 2 : HepG2 (Human liver hepatocellular carcinoma cell line) cell lysates
    Lane 3 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cell lysates
    Lane 4 : BxPC-3 (Human pancreas adenocarcinoma cell line) cell lysates

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 55 kDa



    This image was generated using the unpurified version of the product. 

  • Immunoprecipitation - Anti-xCT antibody [EPR8290(2)] (ab175186)
    Immunoprecipitation - Anti-xCT antibody [EPR8290(2)] (ab175186)

    Western blot analysis on immunoprecipitation pellet from (Lane 1) HepG2 cells lysate or (Lane 2) 1 X PBS (negative control) using ab175186 at a 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

    This image was generated using the unpurified version of the product. 

  • Anti-xCT antibody [EPR8290(2)] (ab175186)
    Anti-xCT antibody [EPR8290(2)] (ab175186)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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