Antibodies, Proteins, Kits and Reagents for Life Science

247 929 ₸ Product size

100 µl 247 929 ₸

Order now and get it on Tuesday March 02, 2021

Anti-S100P antibody [EPR6142] (ab124743)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR6142] to S100P
Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
Reacts with: Human

Product name

Anti-S100P antibody [EPR6142]
See all S100P primary antibodies
Description

Rabbit monoclonal [EPR6142] to S100P
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human S100P aa 1-100. The exact sequence is proprietary.
Positive control
- BxPC3 cell lysate; Human pancreatic adenocarcinoma, placenta and spleen tissues.
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR6142
Isotype

IgG
Research areas

Western blot - Anti-S100P antibody [EPR6142] (ab124743)

Anti-S100P antibody [EPR6142] (ab124743) at 1/1000 dilution (purified) + BxPC-3 cell lysate at 10 µg

Secondary
HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 10 kDa
Observed band size: 10 kDa

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Flow Cytometry - Anti-S100P antibody [EPR6142] (ab124743)

Flow Cytometry analysis of BxPC-3 (human Pancreas adenocarcinoma) cells labeling S100P with purified ab124743 at 1/1700 dilution (1ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100P antibody [EPR6142] (ab124743)

Immunohistochemical staining of paraffin embedded human pancreatic adenocarcinoma with purified ab124743 at a working dilution of 1 in 300. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
Immunocytochemistry/ Immunofluorescence - Anti-S100P antibody [EPR6142] (ab124743)

Immunofluorescence staining of BxPC-3 cells with purified ab124743 at a working dilution of 1 in 150, counter-stained with DAPI. The secondary antibody was Alexa Fluor^® 555 goat anti-rabbit, used at a dilution of 1 in 500. The cells were fixed in 4% PFA. The negative control is shown in bottom right hand panel - for the negative control, purified ab124743 was used at a dilution of 1/200 followed by an Alexa Fluor^® 555 goat anti-mouse antibody at a dilution of 1/500.
Western blot - Anti-S100P antibody [EPR6142] (ab124743)

Anti-S100P antibody [EPR6142] (ab124743) at 1/1000 dilution (unpurified) + BxPC3 cell lysate at 10 µg

Secondary
Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 10 kDa
Observed band size: 10 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100P antibody [EPR6142] (ab124743)

Unpurified ab124743, at 1/500 dilution, staining S100P in paraffin-embedded human pancreatic adenocarcinoma tissue by immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100P antibody [EPR6142] (ab124743)

Unpurified ab124743, at 1/500 dilution, staining S100P in paraffin-embedded human placenta tissue by immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100P antibody [EPR6142] (ab124743)

Unpurified ab124743, at 1/500 dilution, staining S100P in paraffin-embedded human spleen tissue by immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100P antibody [EPR6142] (ab124743)

Unpurified ab124743 showing negative staining in normal liver tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100P antibody [EPR6142] (ab124743)

Unpurified ab124743 showing positive staining in colonic adenocarcinoma tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100P antibody [EPR6142] (ab124743)

Unpurified ab124743 showing negative staining in normal pancreas tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Anti-S100P antibody [EPR6142] (ab124743)

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