Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-S100P antibody [EPR6142] (ab124743)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6142] to S100P
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-S100P antibody [EPR6142]
    See all S100P primary antibodies

  • Description

    Rabbit monoclonal [EPR6142] to S100P

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human S100P aa 1-100. The exact sequence is proprietary.

  • Positive control

    • BxPC3 cell lysate; Human pancreatic adenocarcinoma, placenta and spleen tissues.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-S100P antibody [EPR6142] (ab124743)
    Western blot - Anti-S100P antibody [EPR6142] (ab124743)
    Anti-S100P antibody [EPR6142] (ab124743) at 1/1000 dilution (purified) + BxPC-3 cell lysate at 10 µg

    Secondary
    HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 10 kDa
    Observed band size: 10 kDa



    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Flow Cytometry - Anti-S100P antibody [EPR6142] (ab124743)
    Flow Cytometry - Anti-S100P antibody [EPR6142] (ab124743)

    Flow Cytometry analysis of BxPC-3 (human Pancreas adenocarcinoma) cells labeling S100P with purified ab124743 at 1/1700 dilution (1ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100P antibody [EPR6142] (ab124743)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100P antibody [EPR6142] (ab124743)

    Immunohistochemical staining of paraffin embedded human pancreatic adenocarcinoma with purified ab124743 at a working dilution of 1 in 300. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

  • Immunocytochemistry/ Immunofluorescence - Anti-S100P antibody [EPR6142] (ab124743)
    Immunocytochemistry/ Immunofluorescence - Anti-S100P antibody [EPR6142] (ab124743)

    Immunofluorescence staining of BxPC-3 cells with purified ab124743 at a working dilution of 1 in 150, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 555 goat anti-rabbit, used at a dilution of 1 in 500. The cells were fixed in 4% PFA. The negative control is shown in bottom right hand panel - for the negative control, purified ab124743 was used at a dilution of 1/200 followed by an Alexa Fluor® 555 goat anti-mouse antibody at a dilution of 1/500.

  • Western blot - Anti-S100P antibody [EPR6142] (ab124743)
    Western blot - Anti-S100P antibody [EPR6142] (ab124743)
    Anti-S100P antibody [EPR6142] (ab124743) at 1/1000 dilution (unpurified) + BxPC3 cell lysate at 10 µg

    Secondary
    Goat anti-Rabbit HRP at 1/2000 dilution

    Predicted band size: 10 kDa
    Observed band size: 10 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100P antibody [EPR6142] (ab124743)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100P antibody [EPR6142] (ab124743)

    Unpurified ab124743, at 1/500 dilution, staining S100P in paraffin-embedded human pancreatic adenocarcinoma tissue by immunohistochemistry.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100P antibody [EPR6142] (ab124743)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100P antibody [EPR6142] (ab124743)

    Unpurified ab124743, at 1/500 dilution, staining S100P in paraffin-embedded human placenta tissue by immunohistochemistry.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100P antibody [EPR6142] (ab124743)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100P antibody [EPR6142] (ab124743)

    Unpurified ab124743, at 1/500 dilution, staining S100P in paraffin-embedded human spleen tissue by immunohistochemistry.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100P antibody [EPR6142] (ab124743)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100P antibody [EPR6142] (ab124743)

    Unpurified ab124743 showing negative staining in normal liver tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100P antibody [EPR6142] (ab124743)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100P antibody [EPR6142] (ab124743)

    Unpurified ab124743 showing positive staining in colonic adenocarcinoma tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100P antibody [EPR6142] (ab124743)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100P antibody [EPR6142] (ab124743)

    Unpurified ab124743 showing negative staining in normal pancreas tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Anti-S100P antibody [EPR6142] (ab124743)
    Anti-S100P antibody [EPR6142] (ab124743)

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