Anti-xCT antibody (ab84171)
Key features and details
- Rabbit polyclonal to xCT
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-xCT antibody
See all xCT primary antibodies -
Description
Rabbit polyclonal to xCT -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
-
Immunogen
Synthetic peptide corresponding to Human xCT aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab93079) -
Positive control
- This antibody produced a positive signal in human duodenum membrane tissue lysate.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab84171 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications OrangutanApplication Abreviews Notes WB Notes Application notesWB: Use at a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.Target
-
Function
Sodium-independent, high-affinity exchange of anionic amino acids with high specificity for anionic form of cystine and glutamate. -
Sequence similarities
Belongs to the amino acid-polyamine-organocation (APC) superfamily. L-type amino acid transporter (LAT) (TC 2.A.3.8) family. -
Cellular localization
Membrane. - Information by UniProt
-
Database links
- Entrez Gene: 23657 Human
- Entrez Gene: 100172659 Orangutan
- Omim: 607933 Human
- SwissProt: Q9UPY5 Human
- SwissProt: Q5RAG7 Orangutan
- Unigene: 390594 Human
-
Form
xCT has a predicted molecular weight of 55 kDa; however it has a high number of hydrophobic residues which may affect the migration of the protein in SDS-PAGE. Endogenous monomeric xCT is expected to migrate at ~35 kDa and modified-xCT is expected to migrate at ~55 kDa (PMID: 17035536) -
Alternative names
- Amino acid transport system xc xCT antibody antibody
- Amino acid transport system xc- antibody
- Calcium channel blocker resistance protein CCBR1 antibody
see all
Images
-
Western blot - Anti-xCT antibody (ab84171)Anti-xCT antibody (ab84171) at 1 µg/ml + Human duodenum normal tissue lysate - membrane extract (ab29981) at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 55 kDa
Observed band size: 55 kDa
Exposure time: 10 minutes
Datasheets and documents
References (0)
ab84171 has not yet been referenced specifically in any publications.
Images
-
Western blot - Anti-xCT antibody (ab84171)Anti-xCT antibody (ab84171) at 1 µg/ml + Human duodenum normal tissue lysate - membrane extract (ab29981) at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 55 kDa
Observed band size: 55 kDa
Exposure time: 10 minutes
