Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-UGPase antibody [EPR10627(B)] - BSA and Azide free (ab249123)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10627(B)] to UGPase - BSA and Azide free
  • Suitable for: IHC-P, Flow Cyt, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-UGPase antibody [EPR10627(B)] - BSA and Azide free
    See all UGPase primary antibodies

  • Description

    Rabbit monoclonal [EPR10627(B)] to UGPase - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, Flow Cyt, WBmore details
    Unsuitable for: ICC/IF or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Pig

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab249123 is the carrier-free version of ab154817. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab249123 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-UGPase antibody [EPR10627(B)] - BSA and Azide free (ab249123)
    Western blot - Anti-UGPase antibody [EPR10627(B)] - BSA and Azide free (ab249123)
    All lanes : Anti-UGPase antibody [EPR10627(B)] (ab154817) at 1/1000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : NIH 3T3 cell lysate
    Lane 4 : Human fetal liver tissue lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 57 kDa



    This data was developed using ab154817, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGPase antibody [EPR10627(B)] - BSA and Azide free (ab249123)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGPase antibody [EPR10627(B)] - BSA and Azide free (ab249123)

    This data was developed using ab154817, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human colon adenocarcinoma labeling UGPase using ab154817 at 1/50 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGPase antibody [EPR10627(B)] - BSA and Azide free (ab249123)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGPase antibody [EPR10627(B)] - BSA and Azide free (ab249123)

    This data was developed using ab154817, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human kidney labeling UGPase using ab154817 at 1/50 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Flow Cytometry - Anti-UGPase antibody [EPR10627(B)] - BSA and Azide free (ab249123)
    Flow Cytometry - Anti-UGPase antibody [EPR10627(B)] - BSA and Azide free (ab249123)

    This data was developed using ab154817, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of permeabilized NIH 3T3 cells labeling UGPase using ab154817 (red) at 1/10 dilution or a rabbit IgG (negative) (green).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGPase antibody [EPR10627(B)] - BSA and Azide free (ab249123)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGPase antibody [EPR10627(B)] - BSA and Azide free (ab249123)
    This data was developed using ab154817, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human skeletal muscle tissue using ab154817 showing +ve staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGPase antibody [EPR10627(B)] - BSA and Azide free (ab249123)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGPase antibody [EPR10627(B)] - BSA and Azide free (ab249123)
    This data was developed using ab154817, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human normal liver tissue using ab154817 showing +ve staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGPase antibody [EPR10627(B)] - BSA and Azide free (ab249123)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGPase antibody [EPR10627(B)] - BSA and Azide free (ab249123)
    This data was developed using ab154817, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human normal heart tissue using ab154817 showing +ve staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGPase antibody [EPR10627(B)] - BSA and Azide free (ab249123)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGPase antibody [EPR10627(B)] - BSA and Azide free (ab249123)
    This data was developed using ab154817, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human normal colon tissue using ab154817 showing +ve staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Anti-UGPase antibody [EPR10627(B)] - BSA and Azide free (ab249123)
    Anti-UGPase antibody [EPR10627(B)] - BSA and Azide free (ab249123)

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