Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PMM1 antibody [EPR17844] - BSA and Azide free (ab251358)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17844] to PMM1 - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PMM1 antibody [EPR17844] - BSA and Azide free
    See all PMM1 primary antibodies

  • Description

    Rabbit monoclonal [EPR17844] to PMM1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab251358 is the carrier-free version of ab202058. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251358 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-PMM1 antibody [EPR17844] - BSA and Azide free (ab251358)
    Western blot - Anti-PMM1 antibody [EPR17844] - BSA and Azide free (ab251358)
    All lanes : Anti-PMM1 antibody [EPR17844] (ab202058) at 1/5000 dilution

    Lane 1 : Human fetal liver lysate
    Lane 2 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysate
    Lane 3 : 293 (Human epithelial cells from embryonic kidney) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 30 kDa
    Observed band size: 30 kDa


    Exposure time: 1 minute


    This data was developed using ab202058, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-PMM1 antibody [EPR17844] - BSA and Azide free (ab251358)
    Western blot - Anti-PMM1 antibody [EPR17844] - BSA and Azide free (ab251358)
    All lanes : Anti-PMM1 antibody [EPR17844] (ab202058) at 1/2000 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : Human fetal heart lysate
    Lane 3 : Human fetal kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 30 kDa
    Observed band size: 30 kDa


    Exposure time: 3 minutes


    This data was developed using ab202058, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-PMM1 antibody [EPR17844] - BSA and Azide free (ab251358)
    Western blot - Anti-PMM1 antibody [EPR17844] - BSA and Azide free (ab251358)
    All lanes : Anti-PMM1 antibody [EPR17844] (ab202058) at 1/2000 dilution

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Rat brain tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 30 kDa
    Observed band size: 30 kDa


    Exposure time: 30 seconds


    This data was developed using ab202058, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-PMM1 antibody [EPR17844] - BSA and Azide free (ab251358)
    Western blot - Anti-PMM1 antibody [EPR17844] - BSA and Azide free (ab251358)
    All lanes : Anti-PMM1 antibody [EPR17844] (ab202058) at 1/5000 dilution

    Lane 1 : C6 (Rat glial tumor cells) whole cell lysate
    Lane 2 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
    Lane 3 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 30 kDa
    Observed band size: 30 kDa


    Exposure time: 30 seconds


    This data was developed using ab202058, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMM1 antibody [EPR17844] - BSA and Azide free (ab251358)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMM1 antibody [EPR17844] - BSA and Azide free (ab251358)
    This data was developed using ab202058, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling PMM1 with ab202058 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasmic staining on Human liver tissue is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMM1 antibody [EPR17844] - BSA and Azide free (ab251358)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMM1 antibody [EPR17844] - BSA and Azide free (ab251358)
    This data was developed using ab202058, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Mouse cerebral cortex tissue labeling PMM1 with ab202058 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasmic staining on mouse cerebral cortex is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMM1 antibody [EPR17844] - BSA and Azide free (ab251358)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMM1 antibody [EPR17844] - BSA and Azide free (ab251358)
    This data was developed using ab202058, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded rat pancreas tissue labeling PMM1 with ab202058 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasmic staining on rat pancreas is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Anti-PMM1 antibody [EPR17844] - BSA and Azide free (ab251358)
    Anti-PMM1 antibody [EPR17844] - BSA and Azide free (ab251358)

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