Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Rsk 2 / MAPKAP Kinase 1b antibody [Y83] - BSA and Azide free (ab247236)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [Y83] to Rsk 2 / MAPKAP Kinase 1b - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Rsk 2 / MAPKAP Kinase 1b antibody [Y83] - BSA and Azide free
    See all Rsk 2 / MAPKAP Kinase 1b primary antibodies

  • Description

    Rabbit monoclonal [Y83] to Rsk 2 / MAPKAP Kinase 1b - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    The antibody recognises Rsk2. It does not cross react with other S6 kinase family members.

  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: ICC

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab247236 is the carrier-free version of ab32133. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab247236 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Rsk 2 / MAPKAP Kinase 1b antibody [Y83] - BSA and Azide free (ab247236)
    Western blot - Anti-Rsk 2 / MAPKAP Kinase 1b antibody [Y83] - BSA and Azide free (ab247236)

    This data was developed using ab32133, the same antibody clone in a different buffer formulation.

    Lane 1: Wild-type HAP1 cell lysate (40 µg)

    Lane 2: Rsk 2 / MAPKAP Kinase 1b knockout HAP1 cell lysate (40 µg)

    Lane 3: HeLa cell lysate (40 µg)

    Lane 4: (40 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab32133 observed at 88 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab32133 was shown to specifically react with Rsk 2 / MAPKAP Kinase 1b when Rsk 2 / MAPKAP Kinase 1b knockout samples were used. Wild-type and Rsk 2 / MAPKAP Kinase 1b knockout samples were subjected to SDS-PAGE. ab32133 and ab8245 (loading control to GAPDH) were diluted at 1/5000 and 1:10,000 dilution respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773

    andGoat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776

    secondary antibodies at 1:10,000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rsk 2 / MAPKAP Kinase 1b antibody [Y83] - BSA and Azide free (ab247236)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rsk 2 / MAPKAP Kinase 1b antibody [Y83] - BSA and Azide free (ab247236)
    This data was developed using ab32133, the same antibody clone in a different buffer formulation.ab32133, staining human lymphoma by immunohistochemistry, Paraffin embedded tissue Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Western blot - Anti-Rsk 2 / MAPKAP Kinase 1b antibody [Y83] - BSA and Azide free (ab247236)
    Western blot - Anti-Rsk 2 / MAPKAP Kinase 1b antibody [Y83] - BSA and Azide free (ab247236)
    Anti-Rsk 2 / MAPKAP Kinase 1b antibody [Y83] (ab32133) at 1/5000 dilution + HeLa cell lysate

    Predicted band size: 84 kDa
    Observed band size: 90 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab32133, the same antibody clone in a different buffer formulation.

     

  • Anti-Rsk 2 / MAPKAP Kinase 1b antibody [Y83] - BSA and Azide free (ab247236)
    Anti-Rsk 2 / MAPKAP Kinase 1b antibody [Y83] - BSA and Azide free (ab247236)

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