Astana Biomed Group, an authorized Abcam distributor in Central Asia

Mouse Alanine aminotransferase (ALT) Antibody Pair - BSA and Azide free (ab253647)

Key features and details

  • Unconjugated capture and detector antibodies
  • Adaptable to any antibody pair-based assay format
  • Antibody concentration ~ 1 mg/ml
  • BSA and azide free buffer - ready for conjugation
  • Reacts with: Mouse

Overview

  • Product name

    Mouse Alanine aminotransferase (ALT) Antibody Pair - BSA and Azide free
    See all Alanine Transaminase kits

  • Assay type

    ELISA set

  • Range

    78 pg/ml - 5000 pg/ml

  • Species reactivity

    Reacts with: Mouse

  • Product overview


    • Mouse ALT Antibody Pair is a matched pair of unconjugated recombinant rabbit monoclonal capture and detection antibodies used to quantify Mouse ALT in sandwich ELISAs and many other pair-based applications.

    • The pair can be used in variety of assays and platforms including but not limited to:

    •      - Sandwich ELISA

    •      - FRET/TR-FRET/HTR

    •      - Meso Scale Discovery® (MSD®)

    •      - Luminex® and bead-based assays

    •      - AlphaLISA®/AlphaScreen®

    •      - DELFIA® immunoassays

    •      - Simoa® and Single Molecule Counting (SMC™) immunoassays

    •      - Multiplex

    • Our antibody pairs are supplied in a carrier-free format that is conjugation-ready:

    •      - Buffer free of BSA, sodium azide, and glycerol for higher conjugation efficiency.

    •      - Concentration of ~1 mg/ml as measured by the protein A280 method.

    • Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
    • We can label antibodies for you: get in touch today to discuss how we can help accelerate your assay development with custom conjugation services.

    • Pairs are screened in biological samples, including plasma and serum, to ensure specificity in complex samples.

    • Please note:

    • The recommended antibody orientation is based on internal optimization in sandwich ELISA. Antibody orientation is assay dependent and needs to be optimized for each assay type.

    • The range provided for this antibody pair is based on initial sandwich ELISA validation data using recombinant protein. Performance and range of the antibody pair will depend on the specific characteristics of your assay, including standard protein selection.

    • We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibodies in other assays.

    •  Antibody properties:

    •  Capture antibody: recombinant rabbit monoclonal (unconjugated) – 100 µg

    •  Detector antibody: recombinant rabbit monoclonal (unconjugated) - 100 µg

    •  Concentration: ~1 mg/ml

    •  Storage buffer: 100% PBS

    •  Form: Liquid

    •  Isotype: IgG

    •  Recombinant monoclonal antibodies offer several advantages including:

    •      - High batch-to-batch consistency and reproducibility

    •      - Improved sensitivity and specificity

    •      - Long-term security of supply

    •      - Animal-free production

    • For more information see here.

    • Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    • Meso Scale Discovery and MSD are registered trademarks of Meso Scale Diagnostics, LLC.

    • Luminex is a trademark of Luminex Corporation, registered in the US and other countries.

    • AlphaLISA, AlphaScreen, and DELFIA are registered trademarks of PerkinElmer, Inc.

    • Simoa is a registered trademark of Quanterix, Inc.

    • SMC is a registered trademark of Merck KGaA, Darmstadt, Germany.

  • Tested applications

    Suitable for: Sandwich ELISAmore details

  • Platform

    Reagents

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.

  • Carrier free

    Yes
  • Components Identifier 10 x 96 tests
    Mouse Alanine aminotransferase (ALT) Capture Antibody (unconjugated) —
    Mouse Alanine aminotransferase (ALT) Detector Antibody (unconjugated) —

  • Research areas

  • Function

    Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles.

  • Tissue specificity

    Liver, kidney, heart, and skeletal muscles. Expressed at moderate levels in the adipose tissue.

  • Pathway

    Amino-acid degradation; L-alanine degradation via transaminase pathway; pyruvate from L-alanine: step 1/1.

  • Sequence similarities

    Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Alanine aminotransferase subfamily.

  • Cellular localization

    Cytoplasm.
  • Information by UniProt

  • Alternative names

    • AAT1
    • Alanine aminotransferase
    • Alanine aminotransferase 1
    • ALAT1_HUMAN
    • ALT1
    • Glutamate pyruvate transaminase 1
    • Glutamic alanine transaminase 1
    • Glutamic pyruvate transaminase (alanine aminotransferase)
    • Glutamic pyruvic transaminase 1
    • Glutamic--alanine transaminase 1
    • Glutamic--pyruvic transaminase 1
    • gpt
    • GPT 1
    • GPT1
    see all

  • Database links

    Images

    • Sandwich ELISA - Antibody Pair - BSA and Azide Free (ab253647)
      Sandwich ELISA - Antibody Pair - BSA and Azide Free (ab253647)
      To learn more about the advantages of recombinant antibodies see here.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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