Anti-DLST antibody [EPR13318(B)] (ab177934)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13318(B)] to DLST
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-DLST antibody [EPR13318(B)]
See all DLST primary antibodies -
Description
Rabbit monoclonal [EPR13318(B)] to DLST -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanIP HumanWB Human -
Immunogen
Synthetic peptide within Human DLST aa 200-300 (Cysteine residue). The exact sequence is proprietary.
Database link: P36957 -
Positive control
- HeLa, HepG2, MCF-7 and 293T cell lysates; Human heart and liver tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR13318(B) -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-DLST antibody [EPR13318(B)] (ab177934) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : MCF-7 cell lysate
Lane 4 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 49 kDa
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Immunohistochemical analysis of paraffin-embedded Human heart tissue labeling DLST with ab177934 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling DLST with ab177934 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Western blot analysis on immunoprecipitation pellet from (Lane 1) MCF-7 lysate or (Lane 2) 1XPBS (negative control) using ab177934 and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
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