Anti-P4HA2 antibody [CL0351] (ab211527)
![Anti-P4HA2 antibody [CL0351] (ab211527)](https://www.abcam.com/ps/products/211/ab211527/Images/ab211527-281865-anti-p4ha2-antibody-cl0351-western-blot.jpg "Anti-P4HA2 antibody [CL0351] (ab211527)")
Key features and details
- Mouse monoclonal [CL0351] to P4HA2
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG1
Overview
-
Product name
Anti-P4HA2 antibody [CL0351]
See all P4HA2 primary antibodies -
Description
Mouse monoclonal [CL0351] to P4HA2 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
-
Immunogen
Recombinant fragment corresponding to Human P4HA2 aa 1-200.
Database link: O15460 -
Epitope
Binds to an epitope located within the peptide sequence NTDWPALEDLVLQDS (94-108aa) as determined by overlapping synthetic peptides. -
Positive control
- U251 MG cell line lysate; Human endometrium, testis, duodenum, colorectal cancer and lung cancer tissues.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
CL0351 -
Isotype
IgG1 -
Research areas
Images
-
Western blot - Anti-P4HA2 antibody [CL0351] (ab211527)All lanes : Anti-P4HA2 antibody [CL0351] (ab211527)
Lane 1 : U-251 cells, transfected with control siRNA
Lane 2 : U-251 cells, transfected with target specific siRNA probe #1
Lane 3 : U-251 cells, transfected with target specific siRNA probe #2
Predicted band size: 61 kDaDownregulation of antibody signal confirms target specificity. Remaining % intensity, relative control lane, is indicated. Anti-GAPDH monoclonal antibody was used as loading control.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P4HA2 antibody [CL0351] (ab211527)](https://www.abcam.com/ps/products/211/ab211527/Images/ab211527-259766-anti-p4ha2-antibody-cl0351-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P4HA2 antibody [CL0351] (ab211527)Immunohistochemical analysis of paraffin-embedded Human lung cancer (adenocarcinoma) tissue labeling P4HA2 with ab211527 at 1/200 dilution.
-
![Western blot - Anti-P4HA2 antibody [CL0351] (ab211527)](https://www.abcam.com/ps/products/211/ab211527/Images/ab211527-259761-anti-p4ha2-antibody-cl0351-western-blot.jpg)
Western blot - Anti-P4HA2 antibody [CL0351] (ab211527)Anti-P4HA2 antibody [CL0351] (ab211527) at 1/500 dilution + U251 MG cell line lysate
Predicted band size: 61 kDa
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P4HA2 antibody [CL0351] (ab211527)](https://www.abcam.com/ps/products/211/ab211527/Images/ab211527-259765-anti-p4ha2-antibody-cl0351-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P4HA2 antibody [CL0351] (ab211527)Immunohistochemical analysis of paraffin-embedded Human colorectal cancer tissue labeling P4HA2 with ab211527 at 1/200 dilution.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P4HA2 antibody [CL0351] (ab211527)](https://www.abcam.com/ps/products/211/ab211527/Images/ab211527-259764-anti-p4ha2-antibody-cl0351-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P4HA2 antibody [CL0351] (ab211527)Immunohistochemical analysis of paraffin-embedded Human duodenum tissue labeling P4HA2 with ab211527 at 1/200 dilution.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P4HA2 antibody [CL0351] (ab211527)](https://www.abcam.com/ps/products/211/ab211527/Images/ab211527-259763-anti-p4ha2-antibody-cl0351-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P4HA2 antibody [CL0351] (ab211527)Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling P4HA2 with ab211527 at 1/200 dilution.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P4HA2 antibody [CL0351] (ab211527)](https://www.abcam.com/ps/products/211/ab211527/Images/ab211527-259762-anti-p4ha2-antibody-cl0351-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P4HA2 antibody [CL0351] (ab211527)Immunohistochemical analysis of paraffin-embedded Human endometrium tissue labeling P4HA2 with ab211527 at 1/200 dilution.
